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Literature summary extracted from

  • Wang, C.W.; Oh, M.K.; Liao, J.C.
    Engineered isoprenoid pathway enhances astaxanthin production in Escherichia coli (1999), Biotechnol. Bioeng., 62, 235-241.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.5.1.29 biotechnology reconstruction of the isoprenoid pathway in Escherichia coli. To engineer a host that has the capability to supply geranylgeranyl diphosphate, a common precursor of isoprenoids, isopentenyl diphosphate isomerase (encoded by idi) from Escherichia coli and geranylgeranyl diphosphate synthase (encoded by gps) from Archaeoglobus fulgidus are cloned and overexpressed. The latter is shown to be a multifunctional enzyme converting dimethylallyl diphosphate to geranylgeranyl diphosphate. These two genes and the gene cluster (crtBIYZW) of the marine bacterium Agrobacterium aurantiacum are introduced into Escherichia coli to produce astaxanthin, an orange pigment and antioxidant. The metabolically engineered strain produces astaxanthin at a very high rate Archaeoglobus fulgidus
2.5.1.29 synthesis to engineer a host that has the capability to supply geranylgeranyl diphosphate, a common precursor of isoprenoids, isopentenyl diphosphate isomerase (encoded by idi) from Escherichia coli and geranylgeranyl diphosphate synthase (encoded by gps) from Archaeoglobus fulgidus are cloned and overexpressed. The latter is shown to be a multifunctional enzyme converting dimethylallyl diphosphate to geranylgeranyl diphosphate. These two genes and the gene cluster (crtBIYZW) of the marine bacterium Agrobacterium aurantiacum are introduced into Escherichia coli to produce astaxanthin, an orange pigment and antioxidant. The metabolically engineered strain produces astaxanthin at a very high rate Archaeoglobus fulgidus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.29 to engineer a host that has the capability to supply geranylgeranyl diphosphate, a common precursor of isoprenoids, isopentenyl diphosphate isomerase (encoded by idi) from Escherichia coli and geranylgeranyl diphosphate synthase (encoded by gps) from Archaeoglobus fulgidus are cloned and overexpressed. The latter was shown to be a multifunctional enzyme converting dimethylallyl diphosphate to geranylgeranyl diphosphate. These two genes and the gene cluster (crtBIYZW) of the marine bacterium Agrobacterium aurantiacum are introduced into Escherichia coli to produce astaxanthin, an orange pigment and antioxidant. The metabolically engineered strain produces astaxanthin at a very high rate Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.29 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate Archaeoglobus fulgidus
-
 geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate Archaeoglobus fulgidus
-
 geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate Archaeoglobus fulgidus
-
 geranylgeranyl diphosphate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.29 Archaeoglobus fulgidus Q9V305
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.29 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Archaeoglobus fulgidus geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
 Archaeoglobus fulgidus geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate
-
 Archaeoglobus fulgidus geranylgeranyl diphosphate + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.29 GGPP synthase
-
 Archaeoglobus fulgidus
2.5.1.29 gps
-
 Archaeoglobus fulgidus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.29 37
-
 the enzyme maintains significant activity at 37°C Archaeoglobus fulgidus