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Literature summary extracted from
- Molecular cloning of extremely thermostable esterase gene from hyperthermophilic archaeon Pyrococcus furiosus in Escherichia coli (1998), Biotechnol. Bioeng., 57, 624-629.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.1 | expression in Escherichia coli | Pyrococcus furiosus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.1 | Pyrococcus furiosus | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.1 | 4-methylumbelliferyl acetate + H2O | - |
Pyrococcus furiosus | 4-methylumbelliferone + acetate | - |
? |  |
| 3.1.1.1 | 4-methylumbelliferyl butyrate + H2O | hydrolysis is 50% compared to 4-methylumbelliferyl acetate | Pyrococcus furiosus | 4-methylumbelliferone + butyrate | - |
? | |
| 3.1.1.1 | 4-methylumbelliferyl palmitate + H2O | hydrolysis is 7% compared to 4-methylumbelliferyl acetate | Pyrococcus furiosus | 4-methylumbelliferone + palmitate | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 70 | - |
assay at | Pyrococcus furiosus |
| 3.1.1.1 | 100 | - |
- |
Pyrococcus furiosus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 80 | 110 | 80°C: about 55% of maximal activity, 110°C: about 80% of maximal activity | Pyrococcus furiosus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 100 | - |
half-life: 34 h | Pyrococcus furiosus |
| 3.1.1.1 | 110 | - |
half-life: 6 h | Pyrococcus furiosus |
| 3.1.1.1 | 120 | - |
half-life: 2 h | Pyrococcus furiosus |
| 3.1.1.1 | 126 | - |
half-life: 50 min | Pyrococcus furiosus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 7.6 | - |
- |
Pyrococcus furiosus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 7 | 8.2 | pH 7.0: about 40% of maximal activity, pH 8.2: about 50% of maximal activity | Pyrococcus furiosus |
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