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Literature summary extracted from
- Characterization of H2O-forming NADH oxidase from Streptococcus pyogenes and its application in L-rare sugar production (2012), Bioorg. Med. Chem. Lett., 22, 1931-1935.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.6.3.4 | synthesis | the enzyme may prove to be useful for NAD+ regeneration in the production rare L--sugars such as L-ribose, L-ribulose, and L-xylulose | Streptococcus pyogenes |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.6.3.4 | cloned into the T7 promoter-based plasmid pET28a to give pET28a-SpNox and then heterologously overexpressed in Escherichia coli BL21 (DE3) | Streptococcus pyogenes |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.6.3.4 | C44A | 80% of wild-type activity, production of H2O2 rather than H2O | Streptococcus pyogenes |
| 1.6.3.4 | D292A | mutant enzyme nearly loses all activity, also loses yellow color, indicating that the amino acid is important for activity | Streptococcus pyogenes |
| 1.6.3.4 | G13A | mutant enzyme nearly loses all activity, also loses yellow color, indicating that the amino acid is important for activity | Streptococcus pyogenes |
| 1.6.3.4 | G169A | loses nearly 100% of its activity, indicating that G169 is important for NADH binding | Streptococcus pyogenes |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.3.4 | HgCl2 | - |
Streptococcus pyogenes |  |
| 1.6.3.4 | iodoacetate | - |
Streptococcus pyogenes | |
| 1.6.3.4 | p-chloromercuribenzoate | - |
Streptococcus pyogenes | |
| 1.6.3.4 | Quinine | - |
Streptococcus pyogenes | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.6.3.4 | 0.027 | - |
NADH | pH and temperature not specified in the publication | Streptococcus pyogenes | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.3.4 | additional information | the enzyme does not require a metal cofactor for activity | Streptococcus pyogenes |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.6.3.4 | 50000 | - |
2 * 50000, SDS-PAGE | Streptococcus pyogenes |
| 1.6.3.4 | 100000 | - |
gel filtration | Streptococcus pyogenes |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.6.3.4 | 2 NADH + H+ + O2 | Streptococcus pyogenes | - |
NAD+ + 2 H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.6.3.4 | Streptococcus pyogenes | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.6.3.4 | - |
Streptococcus pyogenes |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.6.3.4 | 2 NADH + H+ + O2 | - |
Streptococcus pyogenes | NAD+ + 2 H2O | - |
? | |
| 1.6.3.4 | 2 NADH + H+ + O2 | no production of H2O2 | Streptococcus pyogenes | NAD+ + 2 H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.6.3.4 | dimer | 2 * 50000, SDS-PAGE | Streptococcus pyogenes |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.6.3.4 | H2O-forming NADH oxidase | - |
Streptococcus pyogenes |
| 1.6.3.4 | SpNox | - |
Streptococcus pyogenes |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.6.3.4 | 55 | - |
- |
Streptococcus pyogenes |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.6.3.4 | 30 | 70 | 30°C: about 75% of maximal activity, 70°C: about 80% of maximal activity | Streptococcus pyogenes |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.6.3.4 | 7 | - |
- |
Streptococcus pyogenes |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.6.3.4 | 6.5 | 8.5 | higher than 70% of the maximum activity at pH values of 6.58.5 | Streptococcus pyogenes |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.3.4 | FAD | contains 0.9 mol of FAD per mol of subunit | Streptococcus pyogenes | |
| 1.6.3.4 | NADH | - |
Streptococcus pyogenes | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.6.3.4 | 11000 | - |
NADH | pH and temperature not specified in the publication | Streptococcus pyogenes | |
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