Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhou, L.; Wu, J.; Janakiraman, V.; Shumilin, I.A.; Bauerle, R.; Kretsinger, R.H.; Woodard, R.W.
    Structure and characterization of the 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Aeropyrum pernix (2012), Bioorg. Chem., 40, 79-86.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.54 expression in Escherichia coli Aeropyrum pernix

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.54 in complex with a manganese ion and phosphoenolpyruvate. Crystals contain a tetramer in the asymmetric unit. A water molecule occupies the presumed binding site for the phosphate group of 4-erythrose 4-phosphate Aeropyrum pernix

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 dipicolinic acid 1 mM, 1 h, 21% residual activity Aeropyrum pernix
2.5.1.54 EDTA 10 mM, 1 h, 21% residual activity Aeropyrum pernix
2.5.1.54 additional information none of the downstream products of the shikimate biosynthetic pathway tested inhibits the activity of the enzyme Aeropyrum pernix

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 0.28
-
 D-erythrose 4-phosphate pH 7.0, 60°C Aeropyrum pernix
2.5.1.54 0.891
-
 phosphoenolpyruvate pH 7.0, 60°C Aeropyrum pernix

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 Zn2+ enzyme contains 0.8 molar equivalents of Zn2+ per subunit Aeropyrum pernix

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.54 29159
-
 2 * 30000, SDS-PAGE, 2 * 29159, calculated Aeropyrum pernix
2.5.1.54 30000
-
 2 * 30000, SDS-PAGE, 2 * 29159, calculated Aeropyrum pernix
2.5.1.54 55400
-
 gel filtration Aeropyrum pernix

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Aeropyrum pernix Q9YEJ7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 recombinant protein Aeropyrum pernix

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Aeropyrum pernix 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.54 homodimer 2 * 30000, SDS-PAGE, 2 * 29159, calculated Aeropyrum pernix

Synonyms

EC Number Synonyms Comment Organism
2.5.1.54 AroG
-
 Aeropyrum pernix
2.5.1.54 DAH7PS
-
 Aeropyrum pernix

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.54 95
-
-
 Aeropyrum pernix

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.54 90
-
 1 h, 50% residual activity Aeropyrum pernix

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.54 1
-
 phosphoenolpyruvate pH 7.0, 60°C Aeropyrum pernix

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 5.7
-
 at 60°C Aeropyrum pernix

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.54 7
-
 60°C, 40% of maximum activity Aeropyrum pernix