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Literature summary extracted from

  • Almrud, J.J.; Dasgupta, R.; Czerwinski, R.M.; Kern, A.D.; Hackert, M.L.; Whitman, C.P.
    Kinetic and structural characterization of DmpI from Helicobacter pylori and Archaeoglobus fulgidus, two 4-oxalocrotonate tautomerase family members (2010), Bioorg. Chem., 38, 252-259.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.2.6
-
 Helicobacter pylori
5.3.2.6
-
 Archaeoglobus fulgidus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.2.6 crystals are grown from 20% dioxane and 50% 2-methyl-2,4-pentanediol, pH 6.0, vapor diffusion method Archaeoglobus fulgidus
5.3.2.6 crystals are grown from 28% polyethylene glycol 400, 200 mM CaCl2, 0.1 M HEPES buffer (pH 7.5), vapor diffusion method Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.2.6 0.09
-
 phenylenolpyruvate pH 7.3, 23°C Archaeoglobus fulgidus
5.3.2.6 0.09
-
 2-hydroxy-2,4-pentadienoate pH 7.3, 23°C Archaeoglobus fulgidus
5.3.2.6 0.11
-
 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate Helicobacter pylori
5.3.2.6 0.11
-
 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to compound 5 Archaeoglobus fulgidus
5.3.2.6 0.135
-
 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to compound 5 Helicobacter pylori
5.3.2.6 0.17
-
 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate Archaeoglobus fulgidus
5.3.2.6 0.19
-
 2-hydroxy-2,4-pentadienoate pH 7.3, 23°C Helicobacter pylori
5.3.2.6 0.23
-
 phenylenolpyruvate pH 7.3, 23°C Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
5.3.2.6 Archaeoglobus fulgidus O29588
-
-
5.3.2.6 Helicobacter pylori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.2.6
-
 Helicobacter pylori
5.3.2.6
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.2.6 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate
-
 Archaeoglobus fulgidus (2E)-5-oxohex-2-enedioate
-
 ?
5.3.2.6 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate
-
 Helicobacter pylori (2E)-5-oxohex-2-enedioic acid
-
 ?
5.3.2.6 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate
-
 Helicobacter pylori (3E)-2-oxohex-3-enedioate
-
 ?
5.3.2.6 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate
-
 Archaeoglobus fulgidus (3E)-2-oxohex-3-enedioate
-
 ?
5.3.2.6 2-hydroxy-2,4-pentadienoate
-
 Helicobacter pylori 2-oxo-4-pentenoate
-
 ?
5.3.2.6 2-hydroxy-2,4-pentadienoate
-
 Archaeoglobus fulgidus 2-oxo-4-pentenoate
-
 ?
5.3.2.6 phenylenolpyruvate
-
 Helicobacter pylori phenylpyruvate
-
 ?
5.3.2.6 phenylenolpyruvate
-
 Archaeoglobus fulgidus phenylpyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.2.6 hexamer the beta–alpha–beta fold is conserved in the monomer Helicobacter pylori
5.3.2.6 hexamer the beta–alpha–beta fold is conserved in the monomer Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
5.3.2.6 4-oxalocrotonate tautomerase
-
 Helicobacter pylori
5.3.2.6 4-oxalocrotonate tautomerase
-
 Archaeoglobus fulgidus
5.3.2.6 AfDmpI
-
 Archaeoglobus fulgidus
5.3.2.6 HpDmpI
-
 Helicobacter pylori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.2.6 23
-
 assay at Helicobacter pylori
5.3.2.6 23
-
 assay at Archaeoglobus fulgidus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.2.6 0.05
-
 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to compound 5 Archaeoglobus fulgidus
5.3.2.6 0.1
-
 2-hydroxy-2,4-pentadienoate pH 7.3, 23°C Archaeoglobus fulgidus
5.3.2.6 0.2
-
 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate Archaeoglobus fulgidus
5.3.2.6 0.6
-
 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate Helicobacter pylori
5.3.2.6 0.6
-
 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to compound 5 Helicobacter pylori
5.3.2.6 2.4
-
 phenylenolpyruvate pH 7.3, 23°C Archaeoglobus fulgidus
5.3.2.6 2.5
-
 2-hydroxy-2,4-pentadienoate pH 7.3, 23°C Helicobacter pylori
5.3.2.6 2.6
-
 phenylenolpyruvate pH 7.3, 23°C Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.2.6 7.3
-
 assay at Helicobacter pylori
5.3.2.6 7.3
-
 assay at Archaeoglobus fulgidus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.2.6 0.45
-
 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to compound 5 Archaeoglobus fulgidus
5.3.2.6 1.1
-
 2-hydroxy-2,4-pentadienoate pH 7.3, 23°C Archaeoglobus fulgidus
5.3.2.6 1.2
-
 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate Archaeoglobus fulgidus
5.3.2.6 4.4
-
 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to compound 5 Helicobacter pylori
5.3.2.6 5.4
-
 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate Helicobacter pylori
5.3.2.6 11
-
 phenylenolpyruvate pH 7.3, 23°C Helicobacter pylori
5.3.2.6 13
-
 2-hydroxy-2,4-pentadienoate pH 7.3, 23°C Helicobacter pylori
5.3.2.6 27
-
 phenylenolpyruvate pH 7.3, 23°C Archaeoglobus fulgidus