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Literature summary extracted from

  • Andreotti, G.; Cubellis, M.V.; Nitti, G.; Sannia, G.; Mai, X.; Adams, M.W.; Marino, G.
    An extremely thermostable aromatic aminotransferase from the hyperthermophilic archaeon Pyrococcus furiosus (1995), Biochim. Biophys. Acta, 1247, 90-96.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.57 0.76
-
 2-oxoglutarate pH 7.6, 80°C Pyrococcus furiosus
2.6.1.57 1.15
-
 L-phenylalanine pH 7.6, 80°C Pyrococcus furiosus
2.6.1.57 1.31
-
 L-tyrosine pH 7.6, 80°C Pyrococcus furiosus
2.6.1.57 2.1
-
 L-tryptophan pH 7.6, 80°C Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.57 44000
-
 2 * 44000, SDS-PAGE Pyrococcus furiosus
2.6.1.57 92000
-
 gel filtration Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.57 Pyrococcus furiosus
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.6.1.57 exposure to 02 does not affect enzyme activity Pyrococcus furiosus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.57
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.57 L-phenylalanine + 2-oxoglutarate
-
 Pyrococcus furiosus phenylpyruvate + L-glutamate
-
 ?
2.6.1.57 L-tryptophan + 2-oxoglutarate
-
 Pyrococcus furiosus 3-indole-2-oxopropanoate + L-glutamate
-
 ?
2.6.1.57 L-tyrosine + 2-oxoglutarate
-
 Pyrococcus furiosus p-hydroxyphenylpyruvate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.57 homodimer 2 * 44000, SDS-PAGE Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
2.6.1.57 ArAT
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.57 95
-
-
 Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.57 additional information
-
 pyridoxal 5'-phosphate and 2-ketoglutarate stabilize the enzyme at extreme temperatures Pyrococcus furiosus
2.6.1.57 80
-
 irreversible denaturation of the pure enzyme is not apparent after 6 h in the presence of pyridoxal 5'-phosphate and 2-ketoglutarate. The apoenzyme (lacking pyridoxal 5'-phosphate) loses 70% of its activity (measured after reconstitution) after 6 h Pyrococcus furiosus
2.6.1.57 95
-
 half-life: 16 h in the presence of pyridoxal 5'-phosphate and 2-ketoglutarate, 12 h if cofactor and substrate are not added Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.57 62
-
 L-tryptophan pH 7.6, 80°C Pyrococcus furiosus
2.6.1.57 72
-
 L-tyrosine pH 7.6, 80°C Pyrococcus furiosus
2.6.1.57 225
-
 2-oxoglutarate pH 7.6, 80°C Pyrococcus furiosus
2.6.1.57 253
-
 L-phenylalanine pH 7.6, 80°C Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.57 pyridoxal 5'-phosphate
-
 Pyrococcus furiosus