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Literature summary extracted from

  • Quartararo, C.E.; Hazra, S.; Hadi, T.; Blanchard, J.S.
    Structural, kinetic and chemical mechanism of isocitrate dehydrogenase-1 from Mycobacterium tuberculosis (2013), Biochemistry, 52, 1765-1775.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.42 gene Rv3339c, expression of N-terminally His6-tagged enzyme Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.42 purified ICDH-1 in complex with NADPH at Mn2+, hanging drop vapor diffusion method, 0.001 ml of protein in 5 mM NADPH, and 5 mM MnCl2 is mixed with 0.001 ml of reservoir solution containing 30% PEG 2000 and 0.1 M Tris-HCl, pH 8.0, room temperature, X-ray diffraction structure determination and analysis at 2.18 A resoltuion Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.42 2-oxoglutarate noncompetitive versus NADP+ Mycobacterium tuberculosis
1.1.1.42 malate noncompetitive versus NADP+ and isocitrate Mycobacterium tuberculosis
1.1.1.42 additional information product inhibition patterns for ICDH-1, overview Mycobacterium tuberculosis
1.1.1.42 NADPH product inhibition competitive versus NADP+ and noncompetitive versus isocitrate Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 additional information
-
 additional information steady-state kinetics, kinetic mechanism of ICDH-1, overview Mycobacterium tuberculosis
1.1.1.42 0.015
-
 NADP+ pH 7.5, temperature not specified in the publication, recombinant His-tagged enzyme Mycobacterium tuberculosis
1.1.1.42 0.05
-
 isocitrate pH 7.5, temperature not specified in the publication, recombinant His-tagged enzyme Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Mn2+ required Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Mycobacterium tuberculosis
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
1.1.1.42 isocitrate + NADP+ Mycobacterium tuberculosis H37Rv
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Mycobacterium tuberculosis P9WKL1 gene Rv3339c
-
1.1.1.42 Mycobacterium tuberculosis H37Rv P9WKL1 gene Rv3339c
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42 recombinant N-terminally His6-tagged enzyme by nickel affinity chromatography and gel filtration Mycobacterium tuberculosis

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.42 isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH + H+ protonation of the enolate to form product 2-oxoglutarate is the rate-limiting step Mycobacterium tuberculosis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
 Mycobacterium tuberculosis 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
1.1.1.42 isocitrate + NADP+
-
 Mycobacterium tuberculosis H37Rv 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
1.1.1.42 additional information Mycobacterium tuberculosis ICDH-1 also catalyzes the formation of 2-hydroxyglutarate Mycobacterium tuberculosis ?
-
 ?
1.1.1.42 additional information Mycobacterium tuberculosis ICDH-1 also catalyzes the formation of 2-hydroxyglutarate Mycobacterium tuberculosis H37Rv ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.42 homodimer each subunit has a Rossmann fold, and a common top domain of interlocking beta sheets Mycobacterium tuberculosis
1.1.1.42 More three-dimensional structure of Mtb ICDH-1, overview Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 ICDH
-
 Mycobacterium tuberculosis
1.1.1.42 ICDH-1
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.42 33
-
 NADP+ pH 7.5, temperature not specified in the publication, recombinant His-tagged enzyme Mycobacterium tuberculosis
1.1.1.42 33
-
 isocitrate pH 7.5, temperature not specified in the publication, recombinant His-tagged enzyme Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.42 7.5
-
 assay at Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+
-
 Mycobacterium tuberculosis