Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Baker, P.; Hillis, C.; Carere, J.; Seah, S.Y.K.
    Protein-protein interactions and substrate channeling in orthologous and chimeric aldolase-dehydrogenase complexes (2012), Biochemistry, 51, 1942-1952.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.87 chimeric complexes of Burkholderia xenovorans and Thermus thermophilus enzymes, TTHB246-BphJ and BphI-TTHB247 created by coexpression of the relevant genes in Escherichia coli using separate expression plasmids Thermus thermophilus
1.2.1.87 separate expression of TTHB247 in recombinant Escherichia coli Thermus thermophilus
4.1.3.43 chimeric complexes of Burkholderia xenovorans and Thermus thermophilus enzymes, TTHB246-BphJ and BphI-TTHB247 created by coexpression of the relevant genes in Escherichia coli using separate expression plasmids Thermus thermophilus
4.1.3.43 separate expression of TTHB246 in recombinant Escherichia coli Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
4.1.3.43 A324G increase of channeling efficiency of propionaldehyde to a value comparable to that of acetaldehyde Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.87 5.5
-
 acetaldehyde app. Km-value, single TTHB247, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 6.4
-
 propionaldehyde app. Km-value, single TTHB247, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 9.4
-
 acetaldehyde app. Km-value, TTHB247-BphI complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 9.6
-
 propionaldehyde app. Km-value, TTHB247-BphI complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 15.4
-
 acetaldehyde app. Km-value, TTHB246-TTHB247 complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 15.7
-
 acetaldehyde app. Km-value, BphJ-TTHB246 complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 16.1
-
 propionaldehyde app. Km-value, BphJ-TTHB246 complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 16.1
-
 propionaldehyde app. Km-value, TTHB246-TTHB247 complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 23.1
-
 propionaldehyde app. Km-value, BphJ-BphI complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 23.6
-
 acetaldehyde app. Km-value, BphJ-BphI complex, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 additional information
-
 additional information with 4-hydroxy-2-oxopentanoate at 50°C Km-value is not affected Thermus thermophilus
4.1.3.43 0.037
-
 4-hydroxy-2-oxohexanoate TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 0.041
-
 4-hydroxy-2-oxopentanoate TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 0.045
-
 4-hydroxy-2-oxopentanoate app. Km-value, with Mn2+ chloride salt, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 0.055
-
 4-hydroxy-2-oxopentanoate app. Km-value, with Co2+ chloride salt, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 0.074
-
 4-hydroxy-2-oxopentanoate app. Km-value, with Ni2+ chloride salt, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 0.08
-
 4-hydroxy-2-oxopentanoate app. Km-value, with Mg2+ chloride salt, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 0.089
-
 4-hydroxy-2-oxopentanoate BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 0.095
-
 4-hydroxy-2-oxopentanoate app. Km-value, with Ca2+ chloride salt, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 0.117
-
 4-hydroxy-2-oxohexanoate BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 0.199
-
 4-hydroxy-2-oxohexanoate BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 0.206
-
 4-hydroxy-2-oxopentanoate single TTHB246, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 0.21
-
 4-hydroxy-2-oxohexanoate single TTHB246, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 0.218
-
 4-hydroxy-2-oxohexanoate TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 0.257
-
 4-hydroxy-2-oxopentanoate TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 0.295
-
 4-hydroxy-2-oxopentanoate BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.87 33000
-
 estimated by SDS-PAGE, in agreement with predicted molecular mass calculated from amino acid sequences Thermus thermophilus
1.2.1.87 33000
-
 native molecular mass of TTHB247, determined by static light scattering Thermus thermophilus
1.2.1.87 40000
-
 native molecular mass of TTHB247, determined by gel filtration Thermus thermophilus
1.2.1.87 137000
-
 molecular mass of BphI-TTHB247 chimeric complex, determined by static light scattering Thermus thermophilus
1.2.1.87 142000
-
 native molecular mass of TTHB246-TTHB247 complex, determined by gel filtration Thermus thermophilus
1.2.1.87 144000
-
 molecular mass of BphI-TTHB247 chimeric complex, determined by gel filtration Thermus thermophilus
1.2.1.87 152000
-
 native molecular mass of TTHB246-TTHB247 complex, determined by static light scattering Thermus thermophilus
4.1.3.43 37000
-
 estimated by SDS-PAGE, in agreement with predicted molecular mass calculated from amino acid sequences Thermus thermophilus
4.1.3.43 72000
-
 native molecular mass of TTHB246, determined by gel filtration Thermus thermophilus
4.1.3.43 78000
-
 native molecular mass of TTHB246, determined by static light scattering Thermus thermophilus
4.1.3.43 142000
-
 native molecular mass of TTHB246-TTHB247 complex, determined by gel filtration Thermus thermophilus
4.1.3.43 144000
-
 molecular mass of TTHB246-BphJ chimeric complex, determined by gel filtration Thermus thermophilus
4.1.3.43 145000
-
 molecular mass of TTHB246-BphJ chimeric complex, determined by static light scattering Thermus thermophilus
4.1.3.43 152000
-
 native molecular mass of TTHB246-TTHB247 complex, determined by static light scattering Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.87 Thermus thermophilus Q53WH9
-
-
4.1.3.43 Thermus thermophilus Q53WI0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.87 purified to homogeneity using Ni2+-NTA chromatography Thermus thermophilus
4.1.3.43 purified to homogeneity using Ni2+-NTA chromatography Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.87 acetaldehyde + CoA + NAD+
-
 Thermus thermophilus acetyl-CoA + NADH + H+
-
 ?
1.2.1.87 propionaldehyde + CoA + NAD+
-
 Thermus thermophilus propanoyl-CoA + NADH + H+
-
 ?
4.1.3.43 (4S)-4-hydroxy-2-oxopentanoate
-
 Thermus thermophilus acetaldehyde + pyruvate
-
 ?
4.1.3.43 4-hydroxy-2-oxohexanoate
-
 Thermus thermophilus propanal + pyruvate
-
 ?
4.1.3.43 4-hydroxy-2-oxopentanoate
-
 Thermus thermophilus acetaldehyde + pyruvate
-
 ?
4.1.3.43 additional information no activity with (4R)-4-hydroxy-2-oxopentanoate Thermus thermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.87 monomer 1 * 33000, SDS-PAGE, enzyme can form a stable heterotetrameric complex with TTHB246 in vitro, consisting of two aldolase and two dehydrogenase subunits Thermus thermophilus
4.1.3.43 dimer enzyme can form a stable heterotetrameric complex with TTHB247 in vitro, consisting of two aldolase and two dehydrogenase subunits Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.2.1.87 TTHB247
-
 Thermus thermophilus
4.1.3.43 TTHB246
-
 Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.87 50
-
 half-life in complex with TTHB246 is 5.9 h Thermus thermophilus
1.2.1.87 50
-
 half-life of chimeric BphI-TTHB247 complex is 0.54 h for BphI activity and 2.47 for TTHB247 activity Thermus thermophilus
1.2.1.87 50
-
 half-life of single enzyme is 1.6 h Thermus thermophilus
4.1.3.43 50
-
 half-life in complex with TTHB247 is 10 h Thermus thermophilus
4.1.3.43 50
-
 half-life of chimeric TTHB246-BphJ complex is 2.66 h for TTHB246 activity and 1.62 for BphJ activity Thermus thermophilus
4.1.3.43 50
-
 half-life of single enzyme is 42 h Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.87 2.2
-
 acetaldehyde BphJ-TTHB246 complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 2.3
-
 propionaldehyde BphJ-TTHB246 complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 2.6
-
 propionaldehyde TTHB247-BphI complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 2.8
-
 acetaldehyde TTHB247-BphI complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 3.5
-
 acetaldehyde TTHB246-TTHB247 complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 3.6
-
 propionaldehyde TTHB246-TTHB247 complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 7.4
-
 acetaldehyde single TTHB247, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 8.4
-
 propionaldehyde single TTHB247, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 16.3
-
 propionaldehyde BphJ-BphI complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 17.2
-
 acetaldehyde BphJ-BphI complex, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 additional information
-
 additional information with 4-hydroxy-2-oxopentanoate at 50°C turnover number is increased approximately 4fold Thermus thermophilus
4.1.3.43 0.08
-
 4-hydroxy-2-oxopentanoate with Ca2+ chloride salt, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 0.13
-
 4-hydroxy-2-oxopentanoate with Mg2+ chloride salt, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 0.5
-
 4-hydroxy-2-oxohexanoate TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 0.57
-
 4-hydroxy-2-oxopentanoate with Mn2+ chloride salt, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 0.6
-
 4-hydroxy-2-oxopentanoate TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 0.79
-
 4-hydroxy-2-oxopentanoate with Ni2+ chloride salt, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 1
-
 4-hydroxy-2-oxopentanoate single TTHB246, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 1
-
 4-hydroxy-2-oxopentanoate with Co2+ chloride salt, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 1.1
-
 4-hydroxy-2-oxohexanoate single TTHB246, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 2.1
-
 4-hydroxy-2-oxohexanoate BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 2.2
-
 4-hydroxy-2-oxohexanoate TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 2.3
-
 4-hydroxy-2-oxopentanoate BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 2.4
-
 4-hydroxy-2-oxopentanoate TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 3.9
-
 4-hydroxy-2-oxohexanoate BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 4.1
-
 4-hydroxy-2-oxopentanoate BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus

General Information

EC Number General Information Comment Organism
1.2.1.87 metabolism together with TTHB246, EC 4.1.3.43, the enzyme forms a bacterial aldolase-dehydrogenase complex catalyzing the last steps in the meta cleavage pathway of aromatic hydrocarbon degradation Thermus thermophilus
4.1.3.43 metabolism together with TTHB247, EC 1.2.1.87, enzyme forms a bacterial aldolase-dehydrogenase complex catalyzing the last steps in the meta cleavage pathway of aromatic hydrocarbon degradation Thermus thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.87 0.14
-
 acetaldehyde BphJ-TTHB246 complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 0.14
-
 propionaldehyde BphJ-TTHB246 complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 0.22
-
 propionaldehyde TTHB246-TTHB247 complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 0.23
-
 acetaldehyde TTHB246-TTHB247 complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 0.27
-
 propionaldehyde TTHB247-BphI complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 0.3
-
 acetaldehyde TTHB247-BphI complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 0.7
-
 propionaldehyde BphJ-BphI complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 0.73
-
 acetaldehyde BphJ-BphI complex, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 1.31
-
 propionaldehyde single TTHB247, pH 8.0, 25°C Thermus thermophilus
1.2.1.87 1.35
-
 acetaldehyde single TTHB247, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 0.8
-
 4-hydroxy-2-oxopentanoate with app. Km-value, with Ca2+ chloride salt, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 1.6
-
 4-hydroxy-2-oxopentanoate with app. Km-value, with Mg2+ chloride salt, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 4.9
-
 4-hydroxy-2-oxopentanoate single TTHB246, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 5.2
-
 4-hydroxy-2-oxohexanoate single TTHB246, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 7.8
-
 4-hydroxy-2-oxopentanoate BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 9.3
-
 4-hydroxy-2-oxopentanoate TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 10.1
-
 4-hydroxy-2-oxohexanoate TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 10.6
-
 4-hydroxy-2-oxohexanoate BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 11
-
 4-hydroxy-2-oxopentanoate with app. Km-value, with Ni2+ chloride salt, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 13
-
 4-hydroxy-2-oxopentanoate with app. Km-value, with Mn2+ chloride salt, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 13.5
-
 4-hydroxy-2-oxohexanoate TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 14.6
-
 4-hydroxy-2-oxopentanoate TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 19
-
 4-hydroxy-2-oxopentanoate with app. Km-value, with Co2+ chloride salt, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 33.3
-
 4-hydroxy-2-oxohexanoate BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus
4.1.3.43 46.1
-
 4-hydroxy-2-oxopentanoate BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C Thermus thermophilus