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Literature summary extracted from

  • Alontaga, A.Y.; Fenton, A.W.
    Effector analogues detect varied allosteric roles for conserved protein-effector interactions in pyruvate kinase isozymes (2011), Biochemistry, 50, 1934-1939.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.40 expression of wild-type and mutant H476L in the FF50 strain of Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.40 H476L site-directed mutagenesis Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 2-aminoisobutyric acid allosteric inhibition Homo sapiens
2.7.1.40 D-alanine allosteric inhibition Homo sapiens
2.7.1.40 L-alanine allosteric inhibition. The pyruvate kinase isozyme from human liver has decreased affinity for phosphoenolpyruvate when allosterically inhibited by alanine. Minimal effect on coupling caused by the methyl group substitution to Ala (2-aminoisobutyric acid vs. Ala) Homo sapiens
2.7.1.40 L-cysteine allosteric inhibition Homo sapiens
2.7.1.40 L-phenylalanine allosteric inhibition. Replacement of the alpha-hydrogen of L-Phe with a methyl group (S)-2-amino-2-methyl-3-phenyl-propionic acid eliminates an allosteric response Homo sapiens
2.7.1.40 L-proline allosteric inhibition Homo sapiens
2.7.1.40 L-valine allosteric inhibition Homo sapiens
2.7.1.40 additional information no inhibition by D-phenylalanine and (S)-2-amino-2-methyl-3-phenyl-propionic acid. Analysis of the binding site for allosteric inhibitor amino acids and the allosteric kinetic mechanism, overview. L-Phe elicits the smallest antagonism of phosphoenolpyruvate affinity of all amino acids tested Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.40 ATP + pyruvate Homo sapiens
-
 ADP + phosphoenolpyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 recombinant wild-type and mutant H476L from the FF50 strain of Escherichia coli by ammonium sulfate fractionation and anion exchange chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 liver liver-specific isozyme hL-PYK Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ATP + pyruvate
-
 Homo sapiens ADP + phosphoenolpyruvate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.40 hL-PYK
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.40 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 7.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.40 ATP
-
 Homo sapiens