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Literature summary extracted from

  • Arnett, S.O.; Gerratana, B.; Townsend, C.A.
    Rate-limiting steps and role of active site Lys443 in the mechanism of carbapenam synthetase (2007), Biochemistry, 46, 9337-9345.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.3.6 expression in Escherichia coli Pectobacterium carotovorum

Protein Variants

EC Number Protein Variants Comment Organism
6.3.3.6 K443A at pH 8.0, 6.33 and 9.33 the mutant enzyme shows no measurable activity Pectobacterium carotovorum
6.3.3.6 K443M at pH 8.0, 6.33 and 9.33 the mutant enzyme shows no measurable activity Pectobacterium carotovorum

Organism

EC Number Organism UniProt Comment Textmining
6.3.3.6 Pectobacterium carotovorum
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-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.3.6 wild-type, K443A and K443M mutant enzymes Pectobacterium carotovorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.3.6 ATP + (2S,5S)-5-carboxymethylproline a mechanism is proposed where the rate-limiting step is beta-lactam ring formation coupled to a protein conformational change. The role of K443 throughout the reaction is undercored Pectobacterium carotovorum AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate
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 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.3.6 CPS
-
 Pectobacterium carotovorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.3.6 25
-
 assay at Pectobacterium carotovorum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.3.6 0.36
-
 (2S,5S)-5-carboxymethylproline pH 8.0, 25°, wild-type enzyme Pectobacterium carotovorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.3.6 8
-
 assay at Pectobacterium carotovorum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.3.3.6 1.04
-
 (2S,5S)-5-carboxymethylproline pH 8.0, 25°, wild-type enzyme Pectobacterium carotovorum