Literature summary extracted from
Menon, A.L.; Hendrix, H.; Hutchins, A.; Verhagen, M.F.; Adams, M.W.
The delta-subunit of pyruvate ferredoxin oxidoreductase from Pyrococcus furiosus is a redox-active, iron-sulfur protein: evidence for an ancestral relationship with 8Fe-type ferredoxins (1998), Biochemistry, 37, 12838-12846.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.2.7.1 |
the gene encoding the delta-subunit is expressed in Escherichia coli |
Pyrococcus furiosus |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.2.7.1 |
Iron |
the enzyme contains at least two [4Fe-4S] clusters. The purified and reconstituted delta subunit contains 8 Fe mol/mol |
Pyrococcus furiosus |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.2.7.1 |
pyruvate + CoA + 2 oxidized ferredoxin |
Pyrococcus furiosus |
the enzyme catalyzes the final oxidative step in carbohydrate fermentation in which pyruvate is oxidized to acetyl-CoA and CO2, coupled to the reduction of ferredoxin |
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.2.7.1 |
Pyrococcus furiosus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.2.7.1 |
recombinantly expressed delta subunit is purified after reconstitution with iron and sulfide. The reconstituted delta-subunit is monomeric with a mass of 11879 Da as determined by mass spectrometry. The purified and reconstituted delta subunit contains 8 Fe mol/mol and remains intact when incubated at 85 °C for 2 h |
Pyrococcus furiosus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.2.7.1 |
pyruvate + CoA + 2 oxidized ferredoxin |
the enzyme catalyzes the final oxidative step in carbohydrate fermentation in which pyruvate is oxidized to acetyl-CoA and CO2, coupled to the reduction of ferredoxin |
Pyrococcus furiosus |
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.2.7.1 |
More |
the enzyme is composed of two catalytic units of molecular mass 120000 Da. Each unit consists of four subunits, alpha/beta/gamma/delta, with masses of approximately 44000 Da, 36000 Da, 20000 Da, and 12000 Da, respectively, and contains at least two [4Fe-4S] clusters. The recombinantly expressed and reconstituted delta subunit is monomeric with a mass of 11879 Da |
Pyrococcus furiosus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.2.7.1 |
POR |
- |
Pyrococcus furiosus |
1.2.7.1 |
pyruvate ferredoxin oxidoreductase |
- |
Pyrococcus furiosus |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
1.2.7.1 |
85 |
- |
the recombinantly expressed, purified and reconstituted delta subunit is stable for 2 h |
Pyrococcus furiosus |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.2.7.1 |
[4Fe-4S]-center |
the enzyme contains at least two [4Fe-4S] clusters. The purified and reconstituted delta subunit contains 8 Fe mol/mol |
Pyrococcus furiosus |
|