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Literature summary extracted from

  • Chalov, S.E.; Voronina, O.L.; Sergienko, O.N.; Lunin, V.G.
    Thermostable DNA-polymerase from the thermophilic archaeon microorganism Archaeoglobus fulgidus VC16 and its features (2003), Biochemistry (Moscow), 68, 301-308.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.7
-
 Archaeoglobus fulgidus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.7 E170A complete loss of the 3'-5'exonuclease activity Archaeoglobus fulgidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.7 KCl at 100 mM KCl, 98%b loss of activity Archaeoglobus fulgidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.7 Mg2+ maximal activity at 4-10 mM Mg2+ Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.7 94000
-
 x * 94000, SDS-PAGE Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Archaeoglobus fulgidus O29753
-
-

Subunits

EC Number Subunits Comment Organism
2.7.7.7 ? x * 94000, SDS-PAGE Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.7 60 78 at 25°C Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.7 95
-
 t1/2: 7 min. Retains 20% of its polymerase activity after 1 h incubation in the absence of substrates Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.7 6.8 7.5
-
 Archaeoglobus fulgidus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.7 6.5 8.8 pH 6.5: about 65% of maximal activity, pH 8.8: about 50% of maximal activity Archaeoglobus fulgidus