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Literature summary extracted from

  • Prabhu, P.R.; Hudson, A.O.
    Identification and partial characterization of an L-tyrosine aminotransferase (TAT) from Arabidopsis thaliana (2010), Biochem. Res. Int., 2010, 549572.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.5 expressed in Escherichia coli as a His-tagged fusion protein Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.5 0.13
-
 2-oxo-3-phenylpropanoate pH 8.2, 30°C Arabidopsis thaliana
2.6.1.5 0.19
-
 2-oxoglutarate pH 8.2, 30°C Arabidopsis thaliana
2.6.1.5 0.19
-
 L-tyrosine pH 8.2, 30°C Arabidopsis thaliana
2.6.1.5 0.22
-
 3-(4-hydroxyphenyl)-2-oxopropanoate pH 8.2, 30°C Arabidopsis thaliana
2.6.1.5 0.84
-
 L-phenylalanine pH 8.2, 30°C Arabidopsis thaliana
2.6.1.5 1.4
-
 L-glutamate pH 8.2, 30°C Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.5 Arabidopsis thaliana Q9LVY1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.5 using Ni-NTA chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.5 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 Arabidopsis thaliana L-tyrosine + 2-oxoglutarate
-
 r
2.6.1.5 L-phenylalanine + 2-oxoglutarate
-
 Arabidopsis thaliana 2-oxo-3-phenylpropanoate + L-glutamate
-
 r
2.6.1.5 L-tyrosine + 2-oxoglutarate best substrate Arabidopsis thaliana 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 r
2.6.1.5 L-tyrosine + 2-oxopentanoate relative activity compared to 2-oxoglutarate: 3% Arabidopsis thaliana 3-(4-hydroxyphenyl)-2-oxopropanoate + (2S)-2-aminopentanoate
-
 ?
2.6.1.5 L-tyrosine + oxaloacetate relative activity compared to 2-oxoglutarate: 75% Arabidopsis thaliana ?
-
 ?
2.6.1.5 L-tyrosine + prephenate relative activity compared to 2-oxoglutarate: 23% Arabidopsis thaliana ?
-
 ?
2.6.1.5 L-tyrosine + pyruvate relative activity compared to 2-oxoglutarate: 40% Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.5 At5g36160
-
 Arabidopsis thaliana
2.6.1.5 TAT
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.5 30
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.5 8.2
-
 assay at Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.6.1.5 metabolism in vivo analysis by functional complementation shows that the gene is able to complement an Escherichia coli with a background of aminotransferase mutations that confers auxotrophy for L-tyrosine and L-phenylalanine Arabidopsis thaliana