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Literature summary extracted from

  • Jones, M.; Perez-Fons, L.; Robertson, F.; Bramley, P.; Fraser, P.
    Functional characterization of long-chain prenyl diphosphate synthases from tomato (2013), Biochem. J., 449, 729-740.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.85 gene SlSPS, recombinant expression in Escherichia coli. In planta, constitutive overexpression of SlSPS elevates the plastoquinone content of immature tobacco leaves Solanum lycopersicum
2.5.1.86 gene SlDPS, recombinant expression in Escherichia coli Solanum lycopersicum

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.85 additional information virus-induced gene silencing. The gene SlSPS is not able to complement silencing of gene SlDPS, encoding a decaprenyl diphosphate synthase, EC 2.5.1.86 Solanum lycopersicum
2.5.1.86 additional information virus-induced gene silencing. The gene SlSPS is not able to complement silencing of gene SlSPS, encoding a solanesyl diphosphate synthase, EC 2.5.1.85 Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.B14 0.00046
-
 geranylgeranyl diphosphate pH 7.5, 30°C Solanum lycopersicum
2.5.1.B14 0.00151
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 30°C Solanum lycopersicum
2.5.1.91 0.00418
-
 (2E,6E,10E)-geranylgeranyl diphosphate pH 7.5, 30°C Solanum lycopersicum
2.5.1.91 0.00863
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 30°C Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.B14 plastid product solanesol is associated with thylakoid membranes Solanum lycopersicum 9536
-
2.5.1.85 chloroplast
-
 Solanum lycopersicum 9507
-
2.5.1.85 additional information solanesyl diphosphate synthase is targeted to the plastid and both solanesol and plastoquinone are associated with thylakoid membranes Solanum lycopersicum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.91 Mg2+ and Mn2+, effective in stimulating the activity Solanum lycopersicum
2.5.1.91 Mn2+ and Mn2+, effective in stimulating the activity Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.91 45443
-
 x * 45443, calculated Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.86 additional information Solanum lycopersicum the enzyme can utilize geranyl, farnesyl or geranylgeranyl diphosphates in the synthesis of C45 and C50 prenyl diphosphates ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.B14 Solanum lycopersicum Q09IV6
-
-
2.5.1.85 Solanum lycopersicum
-
 gene SlSPS
-
2.5.1.86 Solanum lycopersicum
-
 gene SlDPS
-
2.5.1.91 Solanum lycopersicum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.B14 (2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate vmax/Km for (2E,6E)-farnesyl diphosphate is 2.8fold higher than vmax/Km for geranylgeranyl diphosphate Solanum lycopersicum 6 diphosphate + all-trans-nonaprenyl diphosphate
-
 ?
2.5.1.B14 geranylgeranyl diphosphate + 5 isopentenyl diphosphate vmax/Km for (2E,6E)-farnesyl diphosphate is 2.8fold higher than vmax/Km for geranylgeranyl diphosphate Solanum lycopersicum 5 diphosphate + all-trans-nonaprenyl diphosphate
-
 ?
2.5.1.85 geranylgeranyl diphosphate + 5 isopentenyl diphosphate enzyme SlSPS extends the prenyl chain length of the endogenous ubiquinone to nine isoprene units Solanum lycopersicum 5 diphosphate + all-trans-nonaprenyl diphosphate
-
 ?
2.5.1.86 (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate enzyme SlDPS extends the prenyl chain length of the endogenous ubiquinone to ten isoprene units Solanum lycopersicum 7 diphosphate + trans,octacis-decaprenyl diphosphate
-
 ?
2.5.1.86 additional information the enzyme can utilize geranyl, farnesyl or geranylgeranyl diphosphates in the synthesis of C45 and C50 prenyl diphosphates Solanum lycopersicum ?
-
 ?
2.5.1.91 (2E,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate
-
 Solanum lycopersicum 7 diphosphate + decaprenyl diphosphate plus solanesyl diphosphate ?
2.5.1.91 (2E,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Solanum lycopersicum diphosphate + ? main products are decaprenyl diphosphate and solanesyl diphosphate ?
2.5.1.91 geranyl diphosphate + isopentenyl diphosphate
-
 Solanum lycopersicum diphosphate + ? main products are decaprenyl diphosphate and solanesyl diphosphate ?

Subunits

EC Number Subunits Comment Organism
2.5.1.91 ? x * 45443, calculated Solanum lycopersicum

Synonyms

EC Number Synonyms Comment Organism
2.5.1.B14 SPPS
-
 Solanum lycopersicum
2.5.1.B14 SPS
-
 Solanum lycopersicum
2.5.1.85 long-chain prenyl diphosphate synthase
-
 Solanum lycopersicum
2.5.1.85 SlSPS
-
 Solanum lycopersicum
2.5.1.85 solanesyl diphosphate synthase
-
 Solanum lycopersicum
2.5.1.86 decaprenyl diphosphate synthase
-
 Solanum lycopersicum
2.5.1.86 long-chain prenyl diphosphate synthase
-
 Solanum lycopersicum
2.5.1.86 SlDPS
-
 Solanum lycopersicum
2.5.1.91 Dps
-
 Solanum lycopersicum

General Information

EC Number General Information Comment Organism
2.5.1.B14 physiological function when expressed in Escherichia coli, enzyme extends the prenyl chain length of the endogenous ubiquinone to nine isoprene units respectively. In planta, constitutive overexpression elevates the plastoquinone content of immature tobacco leaves. Virus-induced gene silencing shows that the enzyme is necessary for normal chloroplast structure and function. Plants silenced for SPS are photobleached and accumulate phytoene Solanum lycopersicum
2.5.1.85 malfunction plants silenced for SlSPS are photobleached and accumulate phytoene Solanum lycopersicum
2.5.1.85 metabolism requirement for two long-chain prenyl diphosphate synthases in the tomato, i.e. SlSPS and SlDPS, a decaprenyl diphosphate synthase, EC 2.5.1.86 Solanum lycopersicum
2.5.1.85 physiological function enzyme SlSPS is necessary for normal chloroplast structure and function Solanum lycopersicum
2.5.1.86 malfunction plants silenced for SlSPS are not affected in leaf appearance, but impacted on primary metabolism Solanum lycopersicum
2.5.1.86 metabolism requirement for two long-chain prenyl diphosphate synthases in the tomato, i.e. SlSPS, a solanesyl diphosphate synthase, EC 2.5.1.85, and SlDPS, a decaprenyl diphosphate synthase Solanum lycopersicum