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Literature summary extracted from

  • Ogawa, T.; Yoshimura, T.; Hemmi, H.
    Connected cavity structure enables prenyl elongation across the dimer interface in mutated geranylfarnesyl diphosphate synthase from Methanosarcina mazei (2011), Biochem. Biophys. Res. Commun., 409, 333-337.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.81 model structure of mutant I112A, calculated using the model of wild-type GFPS as a template. In wild-type enzyme, the I112 on each subunit is located next to the I112 on the other at the dimer interface, to separate the two reaction cavities. In the mutant enzyme, the substitution of I112 by alanine results in the formation of a large tunnel between dimer subunits, enabling prenyl diphosphates to elongate through the newly-created path from one cavity to the other, thus yielding extremely lengthened prenyl diphosphates Methanosarcina mazei

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.81 D109A mutation leads to the production of longer prenyl diphosphates from C30 to C35, but at small rates Methanosarcina mazei
2.5.1.81 I112A chain length of the products of mutant I112A reaches up to C70 or longer, which is three times as long as that of the wild type. A large fraction of the longer products is C45-50, which is nearly twice as long as wild-type Methanosarcina mazei
2.5.1.81 II116A mutation leads to the production of longer prenyl diphosphates from C30 to C35, but at small rates Methanosarcina mazei
2.5.1.81 M137A slight increase in chain length of products Methanosarcina mazei
2.5.1.81 M72A slight increase in chain length of products Methanosarcina mazei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.81 33836
-
 2 * 33836, calculated Methanosarcina mazei
2.5.1.81 48900
-
 gel filtration Methanosarcina mazei

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.81 Methanosarcina mazei Q8PYS1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.81 geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Methanosarcina mazei (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate among mutants, in which the bulky residues that constitute the bottom of the product-accommodating cavity is replaced with alanine, those having mutations on an alpha-helix existing at the subunit interface yield longer products ?
2.5.1.81 additional information the residues in chain length determination region III are the predominant factor in the determination of the product specificity Methanosarcina mazei ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.81 dimer 2 * 33836, calculated Methanosarcina mazei

Synonyms

EC Number Synonyms Comment Organism
2.5.1.81 GFPS
-
 Methanosarcina mazei