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Literature summary extracted from

  • Olmo-Mira, M.F.; Cabello, P.; Pino, C.; Martinez-Luque, M.; Richardson, D.J.; Castillo, F.; Roldan, M.D.; Moreno-Vivian, C.
    Expression and characterization of the assimilatory NADH-nitrite reductase from the phototrophic bacterium Rhodobacter capsulatus E1F1 (2006), Arch. Microbiol., 186, 339-344.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.15 expressed in Escherichia coli JM109 cells Rhodobacter capsulatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.15 2,2'-dipyridyl 68% inhibition at 2 mM Rhodobacter capsulatus
1.7.1.15 azide 36% inhibition at 2 mM Rhodobacter capsulatus
1.7.1.15 Cyanate 27% inhibition at 2 mM Rhodobacter capsulatus
1.7.1.15 cyanide 69% inhibition at 2 mM Rhodobacter capsulatus
1.7.1.15 dithioerythritol 52% inhibition at 2 mM Rhodobacter capsulatus
1.7.1.15 additional information the activity of the His6-tagged NasB protein is unaffected by thiocyanate Rhodobacter capsulatus
1.7.1.15 o-phenanthroline 72% inhibition at 2 mM Rhodobacter capsulatus
1.7.1.15 p-hydroxymercurybenzoate 48% inhibition at 2 mM Rhodobacter capsulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.15 0.02
-
 NADH at pH 9.0 and 30°C Rhodobacter capsulatus
1.7.1.15 0.5
-
 nitrite at pH 9.0 and 30°C Rhodobacter capsulatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.1.15 Iron contains 2.8 mol iron per mol of protein Rhodobacter capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.15 86600
-
 x * 86600, calculated from amino acid sequence Rhodobacter capsulatus
1.7.1.15 90000
-
 x * 90000, His6-tagged enzyme, SDS-PAGE Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.15 nitrite + NADH + H+ Rhodobacter capsulatus NADH is the best acceptor ammonia + NAD+ + H2O
-
 ?
1.7.1.15 nitrite + NADH + H+ Rhodobacter capsulatus E1F1 NADH is the best acceptor ammonia + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.15 Rhodobacter capsulatus Q6WRS9
-
-
1.7.1.15 Rhodobacter capsulatus E1F1 Q6WRS9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.15 Ni-NTA column chromatography Rhodobacter capsulatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.15 2
-
 purified enzyme, at pH 9.0 and 30°C Rhodobacter capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.15 additional information NADPH is not an effcient electron donor Rhodobacter capsulatus ?
-
 ?
1.7.1.15 additional information NADPH is not an effcient electron donor Rhodobacter capsulatus E1F1 ?
-
 ?
1.7.1.15 nitrite + NADH + H+ NADH is the best acceptor Rhodobacter capsulatus ammonia + NAD+ + H2O
-
 ?
1.7.1.15 nitrite + NADH + H+ NADH is the best acceptor Rhodobacter capsulatus E1F1 ammonia + NAD+ + H2O
-
 ?
1.7.1.15 nitrite + reduced benzyl viologen
-
 Rhodobacter capsulatus ammonia + oxidized benzyl viologen
-
 ?
1.7.1.15 nitrite + reduced benzyl viologen
-
 Rhodobacter capsulatus E1F1 ammonia + oxidized benzyl viologen
-
 ?
1.7.1.15 nitrite + reduced methyl viologen
-
 Rhodobacter capsulatus ammonia + oxidized methyl viologen
-
 ?
1.7.1.15 nitrite + reduced methyl viologen
-
 Rhodobacter capsulatus E1F1 ammonia + oxidized methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.15 ? x * 86600, calculated from amino acid sequence Rhodobacter capsulatus
1.7.1.15 ? x * 90000, His6-tagged enzyme, SDS-PAGE Rhodobacter capsulatus

Synonyms

EC Number Synonyms Comment Organism
1.7.1.15 assimilatory NADH-nitrite reductase
-
 Rhodobacter capsulatus
1.7.1.15 NasB protein
-
 Rhodobacter capsulatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.15 30
-
-
 Rhodobacter capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.15 9
-
-
 Rhodobacter capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.15 FAD
-
 Rhodobacter capsulatus
1.7.1.15 Fe-S center
-
 Rhodobacter capsulatus
1.7.1.15 siroheme
-
 Rhodobacter capsulatus