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Literature summary extracted from

  • Yang, X.; Ma, K.
    Purification and characterization of an NADH oxidase from extremely thermophilic anaerobic bacterium Thermotoga hypogea (2005), Arch. Microbiol., 183, 331-337.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.3.3 5,5'-dithiobis-(2-nitrobenzoate) 1 mM, 7 min, 50% loss of activity Pseudothermotoga hypogea
1.6.3.3 CuSO4 1 mM, 1 h at 22°C, 85% remaining activity Pseudothermotoga hypogea
1.6.3.3 FeSO4 1 mM, 1 h at 22°C, 56% remaining activity Pseudothermotoga hypogea
1.6.3.3 HgCl2 1 mM, 1 h at 22°C, 6% remaining activity Pseudothermotoga hypogea
1.6.3.3 MgCl2 1 mM, 1 h at 22°C, 93% remaining activity Pseudothermotoga hypogea
1.6.3.3 NiCl2 1 mM, 1 h at 22°C, 71% remaining activity Pseudothermotoga hypogea
1.6.3.3 ZnSO4 1 mM, 1 h at 22°C, 69% remaining activity Pseudothermotoga hypogea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.3.3 0.0075
-
 NADH pH 7.0, 80°C Pseudothermotoga hypogea
1.6.3.3 0.085
-
 O2 pH 7.0, 80°C Pseudothermotoga hypogea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.3.3 50000
-
 2 * 50000, SDS-PAGE Pseudothermotoga hypogea
1.6.3.3 100000
-
 gel filtration Pseudothermotoga hypogea

Organism

EC Number Organism UniProt Comment Textmining
1.6.3.3 Pseudothermotoga hypogea
-
-
-
1.6.3.3 Pseudothermotoga hypogea DSM 11164
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.3.3
-
 Pseudothermotoga hypogea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.3.3 30
-
 pH 7.0, 80°C Pseudothermotoga hypogea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.3.3 NADH + H+ + O2 the enzyme is highly specific for NADH. No formation of H2O Pseudothermotoga hypogea NAD+ + H2O2
-
 ?
1.6.3.3 NADH + H+ + O2 the enzyme is highly specific for NADH. No formation of H2O Pseudothermotoga hypogea DSM 11164 NAD+ + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.3.3 homodimer 2 * 50000, SDS-PAGE Pseudothermotoga hypogea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.6.3.3 85
-
 above Pseudothermotoga hypogea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.3.3 70
-
 8 h, no loss of activity Pseudothermotoga hypogea
1.6.3.3 90
-
 100 min, 50% loss of activity Pseudothermotoga hypogea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.3.3 7
-
-
 Pseudothermotoga hypogea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.3.3 6.5 7.5 very active in the pH-range 6.5-7.5 Pseudothermotoga hypogea

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.3.3 FAD a flavoprotein, contains 1.8 mol of non-covalently bound FAD per mol of native enzyme Pseudothermotoga hypogea
1.6.3.3 NADH the enzyme is highly specific for NADH Pseudothermotoga hypogea

General Information

EC Number General Information Comment Organism
1.6.3.3 physiological function further investigation is required to conclude firmly if the purified NADH oxidase is part of an enzyme system that protects anaerobic Thermotoga hypogea from accidental exposure to O2 Pseudothermotoga hypogea