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Literature summary extracted from

  • Yu, X.; Xie, X.; Li, S.M.
    Substrate promiscuity of secondary metabolite enzymes: prenylation of hydroxynaphthalenes by fungal indole prenyltransferases (2011), Appl. Microbiol. Biotechnol., 92, 737-748.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.5.1.80 drug development 7-DMATS catalyzes regio- and stereospecific prenylations and can be used as efficient catalysts for chemoenzymatic synthesis of prenylated compounds, which can be then tested for their biological activities in drug discovery and development programs Aspergillus fumigatus
2.5.1.80 synthesis 7-DMATS catalyzes regio- and stereospecific prenylations and can be used as efficient catalysts for chemoenzymatic synthesis of prenylated compounds Aspergillus fumigatus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.34 recombinant expression Aspergillus fumigatus
2.5.1.80 expression of His-tagged 7-DMATS in Escherichia coli strains XL1 Blue or M15 or in Saccharomyces cerevisiae strain INVSc1 Aspergillus fumigatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.34 Mg2+ required Aspergillus fumigatus
2.5.1.80 Mg2+ required Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.34 dimethylallyl diphosphate + L-tryptophan Aspergillus fumigatus
-
 diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
2.5.1.80 dimethylallyl diphosphate + L-tryptophan Aspergillus fumigatus
-
 diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
2.5.1.122 dimethylallyl diphosphate + L-tyrosine Leptosphaeria maculans
-
 diphosphate + 4-O-dimethylallyl-L-tyrosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.34 Aspergillus fumigatus
-
-
-
2.5.1.80 Aspergillus fumigatus
-
-
-
2.5.1.122 Leptosphaeria maculans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.34 recombinant enzyme Aspergillus fumigatus
2.5.1.80 recombinant His-tagged 7-DMATS from Escherichia coli strains XL1 Blue or M15 or from Saccharomyces cerevisiae strain INVSc1 by nickel affinity chromatography Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.34 dimethylallyl diphosphate + 1-naphthol
-
 Aspergillus fumigatus diphosphate + ?
-
 ?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan
-
 Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan L-tryptophan is the preferred substrate Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
2.5.1.80 dimethylallyl diphosphate + 1,6-dihydroxynaphthalene
-
 Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-en-1-yl)naphthalene-1,6-diol
-
 ?
2.5.1.80 dimethylallyl diphosphate + 1,7-dihydroxynaphthalene
-
 Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-en-1-yl)naphthalene-1,7-diol
-
 ?
2.5.1.80 dimethylallyl diphosphate + 1-naphthol
-
 Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-en-1-yl)naphthalen-1-ol
-
 ?
2.5.1.80 dimethylallyl diphosphate + 2,6-dihydroxynaphthalene
-
 Aspergillus fumigatus diphosphate + 1-(3-methylbut-2-en-1-yl)naphthalene-2,6-diol
-
 ?
2.5.1.80 dimethylallyl diphosphate + 2,7-dihydroxynaphthalene
-
 Aspergillus fumigatus diphosphate + 1-(3-methylbut-2-en-1-yl)naphthalene-2,7-diol + 3-(3-methylbut-2-en-1-yl)naphthalene-2,7-diol + 7-[(3-methylbut-2-en-1-yl)oxy]naphthalen-2-ol + 1,6-bis(3-methylbut-2-en-1-yl)naphthalene-2,7-diol 1-(3-methylbut-2-en-1-yl)naphthalene-2,7-diol is the major product ?
2.5.1.80 dimethylallyl diphosphate + L-tryptophan
-
 Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
2.5.1.80 additional information substrate specificity of 7-DMATS, overview Aspergillus fumigatus ?
-
 ?
2.5.1.122 dimethylallyl diphosphate + L-tyrosine
-
 Leptosphaeria maculans diphosphate + 4-O-dimethylallyl-L-tyrosine
-
 ?
2.5.1.122 additional information no activity with 1-naphthol, 1,6-dihydroxynaphthalene, 1,7-dihydroxynaphthalene, 2,6-dihydroxynaphthalene, 2,7-dihydroxynaphthalene, 3,5-dihydroxy-2-naphthoic acid, and 3,7-dihydroxy-2-naphthoic acid Leptosphaeria maculans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.34 FgaPT2
-
 Aspergillus fumigatus
2.5.1.80 7-dimethylallyltryptophan synthase
-
 Aspergillus fumigatus
2.5.1.80 7-DMATS
-
 Aspergillus fumigatus
2.5.1.122 SirD
-
 Leptosphaeria maculans
2.5.1.122 tyrosine O-prenyltransferase
-
 Leptosphaeria maculans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.34 30
-
 assay at Aspergillus fumigatus
2.5.1.80 30
-
 assay at Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.34 7.5
-
 assay at Aspergillus fumigatus
2.5.1.80 7.5
-
 assay at Aspergillus fumigatus

General Information

EC Number General Information Comment Organism
2.5.1.34 evolution FgaPT2 belongs to the DMATS superfamily Aspergillus fumigatus
2.5.1.80 evolution the enzyme belongs to the DMATS superfamily Aspergillus fumigatus