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Literature summary extracted from

  • Kallnik, V.; Schulz, C.; Schultz, C.; Schweiger, P.; Deppenmeier, U.
    Properties of recombinant Strep-tagged and untagged hyperthermophilic D-arabitol dehydrogenase from Thermotoga maritima (2011), Appl. Microbiol. Biotechnol., 90, 1285-1293.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.250 gene tm0297, located in an operon with tm0298 encoding a mannitol dehydrogenase, phylogenetic analysis, expression of the Strep-tagged enzyme in Escherichia coli Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.250 0.027
-
 NADH with D-ribulose, pH 7.5, 85°C, recombinant Strep-tagged enzyme Thermotoga maritima
1.1.1.250 0.031
-
 NADH with D-ribulose, pH 7.5, 85°C, recombinant detagged enzyme Thermotoga maritima
1.1.1.250 0.059
-
 NAD+ with D-arabinitol, pH 8.5, 85°C, recombinant detagged enzyme Thermotoga maritima
1.1.1.250 0.069
-
 NAD+ with D-arabinitol, pH 8.5, 85°C, recombinant Strep-tagged enzyme Thermotoga maritima
1.1.1.250 2.6
-
 D-arabinitol pH 8.5, 85°C, recombinant detagged enzyme Thermotoga maritima
1.1.1.250 7.1
-
 D-arabinitol pH 8.5, 85°C, recombinant Strep-tagged enzyme Thermotoga maritima
1.1.1.250 7.2
-
 D-ribulose pH 7.5, 85°C, recombinant detagged enzyme Thermotoga maritima
1.1.1.250 13.9
-
 D-ribulose pH 7.5, 85°C, recombinant Strep-tagged enzyme Thermotoga maritima
1.1.1.250 20
-
 D-xylitol pH 8.5, 85°C, recombinant Strep-tagged enzyme Thermotoga maritima
1.1.1.250 21.7
-
 D-xylitol pH 8.5, 85°C, recombinant detagged enzyme Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.250 K+ twofold activation at 60 mM, contributes to the overall stability of the protein and ise not required for catalytic activity Thermotoga maritima
1.1.1.250 Mg2+ twofold activation at 55 mM, contributes to the overall stability of the protein and is not required for catalytic activity Thermotoga maritima
1.1.1.250 additional information no effect on the activity by CaCl2 or NaCl Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.250 27600
-
 6 * 27600, SDS-PAGE Thermotoga maritima
1.1.1.250 164000
-
 native PAGE Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.250 Thermotoga maritima
-
 gene tm0297
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.250 additional information Thermotoga maritima cannot use D-arabitol as a sole carbon source. Optimal growth temperature of Thermotoga maritima is 80°C Thermotoga maritima
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.250 D-arabinitol + NAD+
-
 Thermotoga maritima D-ribulose + NADH + H+
-
 r
1.1.1.250 D-xylitol + NAD+
-
 Thermotoga maritima D-xylulose + NADH + H+
-
 r
1.1.1.250 additional information the enzyme exclusively catalyzed the NAD(H)-dependent oxidoreduction of D-arabitol, D-xylitol, D-ribulose, or D-xylulose Thermotoga maritima ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.250 homohexamer 6 * 27600, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
1.1.1.250 arabitol 2-dehydrogenase
-
 Thermotoga maritima
1.1.1.250 ArDH
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.250 85
-
-
 Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.250 50 85 inactive below 50°C, optimal activity at 85°C Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.250 additional information
-
 the addition of the Strep-tag led to a destabilization of tmArDH resulting in the loss of thermostability Thermotoga maritima
1.1.1.250 85
-
 rapid inactivation of the recombinant Strep-tagged enzyme, while the detagged enzyme retains over 90% activity after 90 min at 85°C Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.250 11
-
 D-arabinitol pH 8.5, 85°C, recombinant Strep-tagged enzyme Thermotoga maritima
1.1.1.250 11.2
-
 D-xylitol pH 8.5, 85°C, recombinant Strep-tagged enzyme Thermotoga maritima
1.1.1.250 16.6
-
 D-xylitol pH 8.5, 85°C, recombinant detagged enzyme Thermotoga maritima
1.1.1.250 18.7
-
 D-arabinitol pH 8.5, 85°C, recombinant detagged enzyme Thermotoga maritima
1.1.1.250 165.6
-
 D-ribulose pH 7.5, 85°C, recombinant Strep-tagged enzyme Thermotoga maritima
1.1.1.250 193.2
-
 D-ribulose pH 7.5, 85°C, recombinant detagged enzyme Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.250 7.5
-
 reduction reaction Thermotoga maritima
1.1.1.250 8.5
-
 oxidation reaction Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.250 4.5 9.5 40% and 10% of maximal activity at pH 5.5 and pH 8.5, no activity below pH 4.5 and above pH 9.5 Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.250 NAD+ dependent on Thermotoga maritima
1.1.1.250 NADH dependent on Thermotoga maritima

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.250 0.56
-
 D-xylitol pH 8.5, 85°C, recombinant Strep-tagged enzyme Thermotoga maritima
1.1.1.250 0.74
-
 D-xylitol pH 8.5, 85°C, recombinant detagged enzyme Thermotoga maritima
1.1.1.250 1.5
-
 D-arabinitol pH 8.5, 85°C, recombinant Strep-tagged enzyme Thermotoga maritima
1.1.1.250 7.2
-
 D-arabinitol pH 8.5, 85°C, recombinant detagged enzyme Thermotoga maritima
1.1.1.250 12
-
 D-ribulose pH 7.5, 85°C, recombinant Strep-tagged enzyme Thermotoga maritima
1.1.1.250 27
-
 D-ribulose pH 7.5, 85°C, recombinant detagged enzyme Thermotoga maritima