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Literature summary extracted from

  • Almendros, M.; Berenguer, J.; Sinisterra, J.
    Thermus thermophilus nucleoside phosphorylases active in the synthesis of nucleoside analogues (2012), Appl. Environ. Microbiol., 78, 3128-3135.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.53
-
 guanosine pH 7.0, 80°C Thermus thermophilus
2.4.2.1 1.01
-
 2'-deoxyguanosine pH 7.0, 80°C Thermus thermophilus
2.4.2.1 2.06
-
 2'-deoxyinosine pH 7.0, 80°C Thermus thermophilus
2.4.2.1 2.08
-
 Inosine pH 7.0, 80°C Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 25415
-
 6 * 25415, calculated Thermus thermophilus
2.4.2.1 156000
-
 sedimentation equilibrium anlysis Thermus thermophilus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.4.2.1 2-methyltetrahydrofuran 50%, 25% residual activity Thermus thermophilus
2.4.2.1 dimethyl sulfoxide 50%, 25% residual activity Thermus thermophilus
2.4.2.1 dimethylformamide 50%, 30% residual activity Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Thermus thermophilus Q72IR2
-
-
2.4.2.1 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 Q72IR2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 2'-deoxyguanosine + phosphate
-
 Thermus thermophilus guanine + 2-deoxy-alpha-D-ribose 1-phosphate 22.5% phosphorolysis of substrate within 30 min ?
2.4.2.1 2'-deoxyguanosine + phosphate
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 guanine + 2-deoxy-alpha-D-ribose 1-phosphate 22.5% phosphorolysis of substrate within 30 min ?
2.4.2.1 2'-deoxyinosine + phosphate
-
 Thermus thermophilus hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate 40.5% phosphorolysis of substrate within 30 min ?
2.4.2.1 2'-deoxyinosine + phosphate
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate 40.5% phosphorolysis of substrate within 30 min ?
2.4.2.1 guanosine + phosphate
-
 Thermus thermophilus guanine + alpha-D-ribose 1-phosphate 8.7% phosphorolysis of substrate within 30 min ?
2.4.2.1 guanosine + phosphate
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 guanine + alpha-D-ribose 1-phosphate 8.7% phosphorolysis of substrate within 30 min ?
2.4.2.1 inosine + phosphate
-
 Thermus thermophilus hypoxanthine + alpha-D-ribose 1-phosphate 19.1% phosphorolysis of substrate within 30 min ?
2.4.2.1 inosine + phosphate
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 hypoxanthine + alpha-D-ribose 1-phosphate 19.1% phosphorolysis of substrate within 30 min ?
2.4.2.1 additional information no substrate: acyclovir, benzimidazole, 2,6-diaminopurine Thermus thermophilus ?
-
 ?
2.4.2.1 additional information no substrate: acyclovir, benzimidazole, 2,6-diaminopurine Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.1 hexamer 6 * 25415, calculated Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.1 80
-
 half-life 132 h Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.1 173
-
 Inosine pH 7.0, 80°C Thermus thermophilus
2.4.2.1 296
-
 guanosine pH 7.0, 80°C Thermus thermophilus
2.4.2.1 627
-
 2'-deoxyinosine pH 7.0, 80°C Thermus thermophilus
2.4.2.1 830
-
 2'-deoxyguanosine pH 7.0, 80°C Thermus thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.1 83.2
-
 Inosine pH 7.0, 80°C Thermus thermophilus
2.4.2.1 304.3
-
 2'-deoxyinosine pH 7.0, 80°C Thermus thermophilus
2.4.2.1 558.8
-
 guanosine pH 7.0, 80°C Thermus thermophilus
2.4.2.1 822.5
-
 2'-deoxyguanosine pH 7.0, 80°C Thermus thermophilus