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Literature summary extracted from

  • Van der Borght, J.; Chen, C.; Hoflack, L.; Van Renterghem, L.; Desmet, T.; Soetaert, W.
    Enzymatic properties and substrate specificity of the trehalose phosphorylase from Caldanaerobacter subterraneus (2011), Appl. Environ. Microbiol., 77, 6939-6944.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.64 expressed in Escherichia coli XL10 Gold cells Caldanaerobacter subterraneus subsp. pacificus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.64 D-glucose strong substrate inhibition, 500 mM D-glucose reduces the enzyme activity to 49.1% of the activity at the saturating concentration of 30 mM Caldanaerobacter subterraneus subsp. pacificus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.64 3.5
-
 D-glucose in 50 mM MES buffer (pH 6.0), at 60°C Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64 20.3
-
 alpha-D-xylose in 50 mM MES buffer (pH 6.0), at 60°C Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64 64.8
-
 alpha-D-arabinose in 50 mM MES buffer (pH 6.0), at 60°C Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64 171.5
-
 alpha-D-galactose in 50 mM MES buffer (pH 6.0), at 60°C Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64 183.4
-
 alpha-L-fucose in 50 mM MES buffer (pH 6.0), at 60°C Caldanaerobacter subterraneus subsp. pacificus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.64 90487
-
 x * 90487, calculated from amino acid sequence Caldanaerobacter subterraneus subsp. pacificus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.64 Caldanaerobacter subterraneus subsp. pacificus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.64 Ni-NTA agarose resin column chromatography Caldanaerobacter subterraneus subsp. pacificus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.64 alpha,alpha-trehalose + phosphate specific substrate Caldanaerobacter subterraneus subsp. pacificus beta-D-glucose 1-phosphate + D-glucose
-
 r
2.4.1.64 beta-D-glucose 1-phosphate + alpha-D-allose 4.5% activity compared to D-glucose Caldanaerobacter subterraneus subsp. pacificus ?
-
 r
2.4.1.64 beta-D-glucose 1-phosphate + alpha-D-arabinose 1.8% activity compared to D-glucose Caldanaerobacter subterraneus subsp. pacificus ?
-
 r
2.4.1.64 beta-D-glucose 1-phosphate + alpha-D-fucose 22% activity compared to D-glucose Caldanaerobacter subterraneus subsp. pacificus ?
-
 r
2.4.1.64 beta-D-glucose 1-phosphate + alpha-D-galactose 83.7% activity compared to D-glucose Caldanaerobacter subterraneus subsp. pacificus ?
-
 r
2.4.1.64 beta-D-glucose 1-phosphate + alpha-D-lyxose 1.3% activity compared to D-glucose Caldanaerobacter subterraneus subsp. pacificus ?
-
 r
2.4.1.64 beta-D-glucose 1-phosphate + alpha-D-mannose 14.1% activity compared to D-glucose Caldanaerobacter subterraneus subsp. pacificus ?
-
 r
2.4.1.64 beta-D-glucose 1-phosphate + alpha-D-xylose 135.2% activity compared to D-glucose Caldanaerobacter subterraneus subsp. pacificus ?
-
 r
2.4.1.64 beta-D-glucose 1-phosphate + alpha-L-fucose 109.2% activity compared to D-glucose Caldanaerobacter subterraneus subsp. pacificus ?
-
 r
2.4.1.64 beta-D-glucose 1-phosphate + beta-L-arabinose 79.9% activity compared to D-glucose Caldanaerobacter subterraneus subsp. pacificus ?
-
 r
2.4.1.64 beta-D-glucose 1-phosphate + D-glucose 100% activity with D-glucose Caldanaerobacter subterraneus subsp. pacificus alpha,alpha-trehalose + phosphate
-
 r
2.4.1.64 additional information the enzyme shows no measurable activity with alpha-D-glucosamine, alpha-D-ribose, D-maltose or D-kojibiose Caldanaerobacter subterraneus subsp. pacificus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.64 ? x * 90487, calculated from amino acid sequence Caldanaerobacter subterraneus subsp. pacificus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.64 inverting trehalose phosphorylase
-
 Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64 trehalose phosphorylase
-
 Caldanaerobacter subterraneus subsp. pacificus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.64 80
-
-
 Caldanaerobacter subterraneus subsp. pacificus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.64 65
-
 no loss of activity after 1 h of incubation at 65°C Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64 72
-
 50% residual activity remains after 1 h of incubation at 72°C Caldanaerobacter subterraneus subsp. pacificus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.64 51.4
-
 alpha-D-arabinose in 50 mM MES buffer (pH 6.0), at 60°C Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64 54
-
 alpha-D-galactose in 50 mM MES buffer (pH 6.0), at 60°C Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64 59
-
 D-glucose in 50 mM MES buffer (pH 6.0), at 60°C Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64 64.3
-
 alpha-D-xylose in 50 mM MES buffer (pH 6.0), at 60°C Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64 68.1
-
 alpha-L-fucose in 50 mM MES buffer (pH 6.0), at 60°C Caldanaerobacter subterraneus subsp. pacificus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.64 5.5
-
 the pH optimum is slightly different for both reaction directions, with a lower optimum (about pH 5.5) for the synthesis reaction Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64 7
-
 the pH optimum is slightly different for both reaction directions, with a higher optimum (pH 7.0) for phosphorolysis Caldanaerobacter subterraneus subsp. pacificus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.64 4.5 7 activity is over 90% in the range of pH 4.5 to 7.0 Caldanaerobacter subterraneus subsp. pacificus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.64 Caldanaerobacter subterraneus subsp. pacificus calculated from amino acid sequence
-
 5.4

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.64 0.315
-
 alpha-D-galactose in 50 mM MES buffer (pH 6.0), at 60°C Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64 0.371
-
 alpha-L-fucose in 50 mM MES buffer (pH 6.0), at 60°C Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64 0.793
-
 alpha-D-arabinose in 50 mM MES buffer (pH 6.0), at 60°C Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64 3.17
-
 alpha-D-xylose in 50 mM MES buffer (pH 6.0), at 60°C Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64 17
-
 D-glucose in 50 mM MES buffer (pH 6.0), at 60°C Caldanaerobacter subterraneus subsp. pacificus