Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sakuno, E.; Yabe, K.; Nakajima, H.
    Involvement of two cytosolic enzymes and a novel intermediate, 5-oxoaverantin, in the pathway from 5-hydroxyaverantin to averufin in aflatoxin biosynthesis (2003), Appl. Environ. Microbiol., 69, 6418-6426.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.352 0.083
-
 5'-hydroxyaverantin in 60 mM potassium phosphate buffer (pH 7.5), at 30°C Aspergillus parasiticus
4.2.1.142 0.02
-
 5'-oxoaverantin pH 7.5, 30°C Aspergillus parasiticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.352 cytosol
-
 Aspergillus parasiticus 5829
-
4.2.1.142 cytosol
-
 Aspergillus parasiticus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.352 28000
-
 2 * 28000, SDS-PAGE Aspergillus parasiticus
1.1.1.352 29600
-
 2 * 29600, calculated from amino acid sequence Aspergillus parasiticus
1.1.1.352 60000
-
 gel filtration Aspergillus parasiticus
4.2.1.142 79000
-
 2 * 79000, SDS-PAGE Aspergillus parasiticus
4.2.1.142 158000
-
 gel filtration Aspergillus parasiticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.352 5'-hydroxyaverantin + NAD+ Aspergillus parasiticus
-
 5'-oxoaverantin + NADH + H+
-
 ?
1.1.1.352 5'-hydroxyaverantin + NAD+ Aspergillus parasiticus NIAH-26
-
 5'-oxoaverantin + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.352 Aspergillus parasiticus
-
-
-
1.1.1.352 Aspergillus parasiticus NIAH-26
-
-
-
4.2.1.142 Aspergillus parasiticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.352 ammonium sulfate precipitation, DE 52 column chromatography, butyl Toyopearl column chromatography, hydroxyapatite column chromatography, Mono Q column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration Aspergillus parasiticus
4.2.1.142
-
 Aspergillus parasiticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.352 mycelium
-
 Aspergillus parasiticus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.352 0.000032
-
 crude enzyme, in 60 mM potassium phosphate buffer (pH 7.5), at 30°C Aspergillus parasiticus
1.1.1.352 0.0222
-
 after 694fold purification, in 60 mM potassium phosphate buffer (pH 7.5), at 30°C Aspergillus parasiticus
4.2.1.142 0.0836
-
 pH 7.5, 30°C Aspergillus parasiticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.352 5'-hydroxyaverantin + NAD+
-
 Aspergillus parasiticus 5'-oxoaverantin + NADH + H+
-
 ?
1.1.1.352 5'-hydroxyaverantin + NAD+
-
 Aspergillus parasiticus NIAH-26 5'-oxoaverantin + NADH + H+
-
 ?
4.2.1.142 5'-oxoaverantin i.e. 1,3,6,8-tetrahydroxy-2-[(1S)-1-hydroxy-5-oxohexyl]anthracene-9,10-dione Aspergillus parasiticus (2'S,5'S)-averufin + H2O i.e. 7,9,11-trihydroxy-2-methyl-3,4,5,6-tetrahydro-2,6-epoxy-2H-anthra[2,3-b]oxocin-8,13-dione ?
4.2.1.142 5'-oxoaverantin i.e. 1,3,6,8-tetrahydroxy-2-[(1S)-1-hydroxy-5-oxohexyl]anthracene-9,10-dione Aspergillus parasiticus NIAH-26 (2'S,5'S)-averufin + H2O i.e. 7,9,11-trihydroxy-2-methyl-3,4,5,6-tetrahydro-2,6-epoxy-2H-anthra[2,3-b]oxocin-8,13-dione ?

Subunits

EC Number Subunits Comment Organism
1.1.1.352 homodimer 2 * 28000, SDS-PAGE Aspergillus parasiticus
1.1.1.352 homodimer 2 * 29600, calculated from amino acid sequence Aspergillus parasiticus
4.2.1.142 dimer 2 * 79000, SDS-PAGE Aspergillus parasiticus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.352 AdhA
-
 Aspergillus parasiticus
1.1.1.352 HAVN dehydrogenase
-
 Aspergillus parasiticus
4.2.1.142 OAVN cyclase
-
 Aspergillus parasiticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.352 30 35
-
 Aspergillus parasiticus
4.2.1.142 25 45 broad Aspergillus parasiticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.352 20 40
-
 Aspergillus parasiticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.352 7.5 8.5
-
 Aspergillus parasiticus
4.2.1.142 6 7
-
 Aspergillus parasiticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.352 NAD+ required for activity Aspergillus parasiticus
4.2.1.142 additional information no requirement for NAD(H) or NADP(H) Aspergillus parasiticus