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Literature summary extracted from

  • Dong, G.; Vieille, C.; Savchenko, A.; Zeikus, J.
    Cloning, sequencing, and expression of the gene encoding extracellular alpha-amylase from Pyrococcus furiosus and biochemical characterization of the recombinant enzyme (1997), Appl. Environ. Microbiol., 63, 3569-3576.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 expression in Escherichia coli Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.1 extracellular
-
 Pyrococcus furiosus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 additional information the enzyme does not require Ca2+ for activity Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 66000
-
 2 * 66000, SDS-PAGE Pyrococcus furiosus
3.2.1.1 100000
-
 gel filtration Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Pyrococcus furiosus O08452
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1
-
 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 3900
-
 pH 6.0, at 98°C Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 amylopectin + H2O liquefying enzyme. The main products of polysaccharide hydrolysis are G2 to G7. A small amount of G1 is formed after long hydrolysis periods. The enzyme A hydrolyzes long-chain oligosaccharides faster than shorter chain oligosaccharides Pyrococcus furiosus ?
-
 ?
3.2.1.1 amylose + H2O liquefying enzyme. The main products of polysaccharide hydrolysis are G2 to G7. A small amount of G1 is formed after long hydrolysis periods. The enzyme A hydrolyzes long-chain oligosaccharides faster than shorter chain oligosaccharides Pyrococcus furiosus ?
-
 ?
3.2.1.1 glycogen + H2O liquefying enzyme. The main products of polysaccharide hydrolysis are G2 to G7. A small amount of G1 is formed after long hydrolysis periods. The enzyme A hydrolyzes long-chain oligosaccharides faster than shorter chain oligosaccharides Pyrococcus furiosus ?
-
 ?
3.2.1.1 additional information does not hydrolyze pullulan, cyclodextrins, sucrose Pyrococcus furiosus ?
-
 ?
3.2.1.1 soluble starch + H2O liquefying enzyme. The main products of polysaccharide hydrolysis are G2 to G7. A small amount of G1 is formed after long hydrolysis periods. The enzyme A hydrolyzes long-chain oligosaccharides faster than shorter chain oligosaccharides Pyrococcus furiosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 homodimer 2 * 66000, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 22
-
 no activity at room temperature Pyrococcus furiosus
3.2.1.1 98
-
 assay at Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 98
-
 half-life: 13 min Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 5.5 6
-
 Pyrococcus furiosus
3.2.1.1 6
-
 assay at Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.1 4.7 7 80% activity or more between pH 4.7 and pH 7.0 Pyrococcus furiosus