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Literature summary extracted from

  • Brown, S.H.; Costantino, H.R.; Kelly, R.M.
    Characterization of amylolytic enzyme activities associated with the hyperthermophilic archaebacterium Pyrococcus furiosus (1990), Appl. Environ. Microbiol., 56, 1985-1991.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 alpha-cyclodextrin 1-10 mM, 25-35% inhibition Pyrococcus furiosus
3.2.1.1 beta-cyclodextrin 1-10 mM, 10-25% inhibition Pyrococcus furiosus
3.2.1.1 EDTA 1-10 mM, about 60-65% inhibition Pyrococcus furiosus
3.2.1.1 EGTA 1-10 mM, about 50% inhibition Pyrococcus furiosus
3.2.1.41 alpha-cyclodextrin 1-10 mM, about 85% inhibition Pyrococcus furiosus
3.2.1.41 beta-cyclodextrin 1-10 mM, 80-85% inhibition Pyrococcus furiosus
3.2.1.41 EDTA 1-10 mM, about 70% inhibition Pyrococcus furiosus
3.2.1.41 EGTA 1-10 mM, 65-70% inhibition Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.1 extracellular alpha-amylase is primarily extracellular Pyrococcus furiosus
-
-
3.2.1.1 intracellular alpha-amylase is primarily extracellular Pyrococcus furiosus 5622
-
3.2.1.20 extracellular primarily an intracellular enzyme Pyrococcus furiosus
-
-
3.2.1.20 intracellular primarily an intracellular enzyme Pyrococcus furiosus 5622
-
3.2.1.41 extracellular
-
 Pyrococcus furiosus
-
-
3.2.1.41 intracellular
-
 Pyrococcus furiosus 5622
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Pyrococcus furiosus
-
-
-
3.2.1.20 Pyrococcus furiosus
-
-
-
3.2.1.41 Pyrococcus furiosus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 starch + H2O
-
 Pyrococcus furiosus ?
-
 ?
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.41 pullulan + H2O
-
 Pyrococcus furiosus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 98
-
 assay at Pyrococcus furiosus
3.2.1.41 102 108
-
 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1 90 109 activity at 90°C is about 60% compared to the activity at 108°C Pyrococcus furiosus
3.2.1.20 90 108 activity at 90°C is about 45% of the activity at 108°C Pyrococcus furiosus
3.2.1.41 90 108 90°C: about 60% of maximal activity, 102-108: maximal activity Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 98
-
 8 h, about 70% loss of activity Pyrococcus furiosus
3.2.1.1 105
-
 2 h, about 80% loss of activity Pyrococcus furiosus
3.2.1.20 85
-
 half-life: more than 24 h Pyrococcus furiosus
3.2.1.20 98
-
 half-life: about 10 h Pyrococcus furiosus
3.2.1.20 105
-
 half-life: about 1 h Pyrococcus furiosus
3.2.1.41 85
-
 24 h, about 20% loss of activity Pyrococcus furiosus
3.2.1.41 98
-
 8 h, about 60% loss of activity Pyrococcus furiosus
3.2.1.41 105
-
 2 h, about 80% loss of activity Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 5.6
-
-
 Pyrococcus furiosus
3.2.1.20 5
-
-
 Pyrococcus furiosus
3.2.1.41 5.6
-
-
 Pyrococcus furiosus