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Literature summary extracted from

  • Mann, S.; Eveleigh, L.; Lequin, O.; Ploux, O.
    A microplate fluorescence assay for DAPA aminotransferase by detection of the vicinal diamine 7,8-diaminopelargonic acid (2013), Anal. Biochem., 432, 90-96.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.6.1.62 analysis simple, cheap, and sensitive microplate fluorescence assay for 7,8-diaminopelargonic acid aminotransferase activity, allowing linear detection of 7,8-diaminopelargonic acid in the range of 20 nM to 50 microM. The method relies on the direct detection in the enzymatic reaction mixture of the vicinal diamine 7,8-diaminopelargonic acid derivatized with ortho-phthalaldehyde and 2-mercaptoethanol. The assay was validated with the known inhibitor desmethyl-KAPA, i.e. 8-amino-7-oxopelargonic acid, and adapted to microplate for high-throughput screening Mycobacterium tuberculosis
2.6.1.62 analysis simple, cheap, and sensitive microplate fluorescence assay, allowing linear detection of 7,8-diaminopelargonic acid in the range of 20 nM to 50 microM. The principle of the method is the direct detection in the enzymatic reaction mixture of the vicinal diamine 7,8-diaminopelargonic acid derivatized with ortho-phthalaldehyde and 2-mercaptoethanol. The assay is validated with inhibitor 8-amino-7-oxopelargonic acid and adapted to microplate Mycobacterium tuberculosis
2.6.1.62 analysis microplate fluorescence assay for 7,8-diaminopelargonic acid aminotransferase that is simple, cheap, and sensitive, allowing linear detection of 7,8-diaminopelargonic acid in the range of 20 nM to 50 microM. The principle of the method is the direct detection in the enzymatic reaction mixture of the vicinal diamine 7,8-diaminopelargonic acid derivatized with ortho-phthalaldehyde and 2-mercaptoethanol Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.62 8-amino-7-oxooctanoic acid
-
 Mycobacterium tuberculosis
2.6.1.62 8-amino-7-oxopelargonic acid
-
 Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.62 Mycobacterium tuberculosis
-
-
-
2.6.1.62 Mycobacterium tuberculosis P9WQ81
-
-
2.6.1.62 Mycobacterium tuberculosis ATCC 25618 P9WQ81
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate
-
 Mycobacterium tuberculosis S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate
-
 Mycobacterium tuberculosis ATCC 25618 S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.62 7,8-diaminopelargonic acid aminotransferase
-
 Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.62 0.0015
-
 8-amino-7-oxooctanoic acid Kic value, pH 9.4, 25°C Mycobacterium tuberculosis
2.6.1.62 0.015
-
 8-amino-7-oxooctanoic acid Kiu value, pH 9.4, 25°C Mycobacterium tuberculosis