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Literature summary extracted from

  • Li, D.F.; Zhang, J.Y.; Hou, Y.; Liu, L.; Liu, S.J.; Liu, W.
    Crystallization and preliminary crystallographic analysis of 2-aminophenol 1,6-dioxygenase complexed with substrate and with an inhibitor (2012), Acta Crystallogr. Sect. F, 68, 1337-1340.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.74 holoenzyme, with FeII and in complexes with the substrate 2-aminophenol and the suicide inhibitor 4-nitrocatechol, hanging drop vapor diffusion method, using 0.1 M sodium cacodylate pH 6.5, 25% (w/v) PEG 3350, 0.2 M sodium chloride Comamonas testosteroni

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.74 4-nitrocatechol suicide inhibitor Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.74 29000
-
 2 * 35000 + 2 * 29000, calculated from amino acid sequence Comamonas testosteroni
1.13.11.74 30000
-
 2 * 35000 + 2 * 30000, SDS-PAGE Comamonas testosteroni
1.13.11.74 35000
-
 2 * 35000 + 2 * 29000, calculated from amino acid sequence Comamonas testosteroni
1.13.11.74 35000
-
 2 * 35000 + 2 * 30000, SDS-PAGE Comamonas testosteroni
1.13.11.74 140000
-
 gel filtration Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.74 2-aminophenol + O2 Comamonas testosteroni
-
 2-aminomuconate 6-semialdehyde
-
 ?
1.13.11.74 2-aminophenol + O2 Comamonas testosteroni CNB-1
-
 2-aminomuconate 6-semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.74 Comamonas testosteroni Q6J1Z5 and Q6J1Z6 subunit alpha and catalytic subunit beta
-
1.13.11.74 Comamonas testosteroni CNB-1 Q6J1Z5 and Q6J1Z6 subunit alpha and catalytic subunit beta
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.74 ammonium sulfate precipitation, Mono Q column chromatography, and Superdex 200 gel filtration Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.74 2-aminophenol + O2
-
 Comamonas testosteroni 2-aminomuconate 6-semialdehyde
-
 ?
1.13.11.74 2-aminophenol + O2
-
 Comamonas testosteroni CNB-1 2-aminomuconate 6-semialdehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.74 heterotetramer 2 * 35000 + 2 * 29000, calculated from amino acid sequence Comamonas testosteroni
1.13.11.74 heterotetramer 2 * 35000 + 2 * 30000, SDS-PAGE Comamonas testosteroni

Synonyms

EC Number Synonyms Comment Organism
1.13.11.74 APD
-
 Comamonas testosteroni