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Literature summary extracted from

  • Li, d.e..F.; Zhang, J.Y.; Hou, Y.J.; Liu, L.; Hu, Y.; Liu, S.J.; Wang, d.a..C.; Liu, W.
    Structures of aminophenol dioxygenase in complex with intermediate, product and inhibitor (2013), Acta Crystallogr. Sect. D, 69, 32-43.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.74 expressed in Escherichia coli BL21(DE3) cells Comamonas testosteroni

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.74 in complex with (4Z,6Z)-3-iminooxepin-2(3H)-one, 2-aminomuconic 6-semialdehyde and 4-nitrocatechol, hanging drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.2 mM sodium chloride, 0.1 M sodium cacodylate pH 6.5 Comamonas testosteroni

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.74 E251A inactive Comamonas testosteroni
1.13.11.74 H13A inactive Comamonas testosteroni
1.13.11.74 H195Q inactive Comamonas testosteroni
1.13.11.74 H62A inactive Comamonas testosteroni
1.13.11.74 Y129F inactive Comamonas testosteroni

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.74 4-nitrocatechol suicide inhibitor, the enzyme completely loses its activity towards 2-aminophenol after incubation with 4-nitrocatechol for 100 s Comamonas testosteroni
1.13.11.74 catechol the enzyme completely loses its activity towards 2-aminophenol after incubation with catechol for 100 s Comamonas testosteroni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.74 0.0171
-
2-Aminophenol at pH 8.0, temperature not specified in the publication Comamonas testosteroni
1.13.11.74 0.0775
-
O2 at pH 8.0, temperature not specified in the publication Comamonas testosteroni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.74 Fe2+ the enzyme contains Fe2+ Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.74 2-aminophenol + O2 Comamonas testosteroni
-
2-aminomuconate 6-semialdehyde
-
?
1.13.11.74 2-aminophenol + O2 Comamonas testosteroni CNB-1
-
2-aminomuconate 6-semialdehyde
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.74 Comamonas testosteroni
-
-
-
1.13.11.74 Comamonas testosteroni CNB-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.74
-
Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.74 2-aminophenol + O2
-
Comamonas testosteroni 2-aminomuconate 6-semialdehyde
-
?
1.13.11.74 2-aminophenol + O2
-
Comamonas testosteroni CNB-1 2-aminomuconate 6-semialdehyde
-
?

Subunits

EC Number Subunits Comment Organism
1.13.11.74 heterotetramer
-
Comamonas testosteroni

Synonyms

EC Number Synonyms Comment Organism
1.13.11.74 APD
-
Comamonas testosteroni