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Literature summary extracted from

  • Timofeev, V.; Smirnova, E.; Chupova, L.; Esipov, R.; Kuranova, I.
    X-ray study of the conformational changes in the molecule of phosphopantetheine adenylyltransferase from Mycobacterium tuberculosis during the catalyzed reaction (2012), Acta Crystallogr. Sect. D, 68, 1660-1670.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.3 expressed in Escherichia coli ER2566 cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.3 apo form and in complex with ATP, counter-diffusion method, using 40 mM cacodylate buffer pH 5.5 containing 10 mM MgCl2, 0.15 mM NaCl, 20 mM cobalt hexamine and 15% (w/v) 2-methyl-2,4-pentanediol as precipitant solution Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.3 Mycobacterium tuberculosis P9WPA5
-
-
2.7.7.3 Mycobacterium tuberculosis H37Rv P9WPA5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.3 Q Sepharose XL resin column chromatography, Q Sepharose HP resin column chromatography, and Sepharose S-200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.3 ATP + pantetheine 4'-phosphate
-
 Mycobacterium tuberculosis diphosphate + 3'-dephospho-CoA
-
 r
2.7.7.3 ATP + pantetheine 4'-phosphate
-
 Mycobacterium tuberculosis H37Rv diphosphate + 3'-dephospho-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.3 hexamer x-ray crystallography Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.7.7.3 phosphopantetheine adenylyltransferase
-
 Mycobacterium tuberculosis
2.7.7.3 PPAT
-
 Mycobacterium tuberculosis