Literature summary extracted from

Goldman, S.; Kim, R.; Hung, L.W.; Jancarik, J.; Kim, S.H.
Purification, crystallization and preliminary X-ray crystallographic analysis of Pyrococcus furiosus DNA polymerase (1989), Acta Crystallogr. Sect. D, 54, 986-988.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.7.7.7	overexpressed in Escherichia coli	Pyrococcus furiosus

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.7.7.7	the enzyme is crystallized from 0.08 M ammonium sulfate, 0.05 M Na-cacodylate, pH 6.5, 0.15%(v/v) NP40, 0.05%(v/v) Tween 20 and 4.5%(w/v) polyethylene glycol 6000 by the vapour-diffusion method. The orthorhombic crystals have unit-cell dimensions of a = 92.5, b = 125.4, c = 192.1 A; alpha = beta = gamma = 90 degrees. The crystals diffract beyond 4 A on a 1.08 A synchrotron radiation source	Pyrococcus furiosus

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.7.7	Pyrococcus furiosus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.7.7	-	Pyrococcus furiosus

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Release 2023.1 (January 2023)