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Literature summary extracted from

  • Hamdi, M.; Yoshinaga, M.; Packianathan, C.; Qin, J.; Hallauer, J.; McDermott, J.R.; Yang, H.C.; Tsai, K.J.; Liu, Z.
    Identification of an S-adenosylmethionine (SAM) dependent arsenic methyltransferase in Danio rerio (2012), Toxicol. Appl. Pharmacol., 262, 185-193.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.137 expressed in Escherichia coli BL21 cells Danio rerio

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.137 C165S inactive Danio rerio
2.1.1.137 C210S inactive Danio rerio

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.137 arsenite the enzyme activity is completely inhibited by elevated concentrations of the substrate arsenite (0.2 mM) Danio rerio
2.1.1.137 Selenite addition of selenite at 1:10 molar ratio with arsenite in the reaction mixture completely abolishes the arsenic methylation Danio rerio

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.137 80000
-
 x * 80000, SDS-PAGE Danio rerio

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.137 Danio rerio
-
 line ZF-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.137 Ni-NTA column chromatography Danio rerio

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.137 brain
-
 Danio rerio
-
2.1.1.137 eye
-
 Danio rerio
-
2.1.1.137 gill
-
 Danio rerio
-
2.1.1.137 heart
-
 Danio rerio
-
2.1.1.137 intestine prominent expression in intestine Danio rerio
-
2.1.1.137 kidney
-
 Danio rerio
-
2.1.1.137 liver most prominent expression in liver Danio rerio
-
2.1.1.137 additional information the enzyme is universally expressed in zebrafish tissues Danio rerio
-
2.1.1.137 muscle
-
 Danio rerio
-
2.1.1.137 skin
-
 Danio rerio
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.137 S-adenosyl-L-methionine + arsenite the enzyme methylates arsenite to dimethylarsinic acid as an end product and produces monomethylarsonous acid and monomethylarsonic acid as intermediates Danio rerio S-adenosyl-L-homocysteine + monomethylarsonous acid + monomethylarsonic acid + dimethylarsinic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.137 ? x * 80000, SDS-PAGE Danio rerio

Synonyms

EC Number Synonyms Comment Organism
2.1.1.137 AS3MT
-
 Danio rerio
2.1.1.137 S-adenosylmethionine dependent arsenic methyltransferase
-
 Danio rerio