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Literature summary extracted from

  • Doukov, T.; Seravalli, J.; Stezowski, J.; Ragsdale, S.
    Crystal structure of a methyltetrahydrofolate- and corrinoid-dependent methyltransferase (2000), Structure, 8, 817-830.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.258 expressed in Escherichia coli B834 cells Moorella thermoacetica

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.258 hanging drop vapor diffusion method, using 9-15% (w/v) polyethylene glycol monomethyl ester 5000, 20-50 mM CaCl2, 20% (v/v) glycerol, and 50 mM HEPES, pH 7.5 Moorella thermoacetica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.258 28000
-
 2 * 28000 Moorella thermoacetica

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.258 Moorella thermoacetica Q46389 formerly Clostridium thermoaceticum
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.258 phenyl Sepharose column chromatography and Q-Sepharose column chromatography Moorella thermoacetica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.258 [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrofolate
-
 Moorella thermoacetica [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrofolate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.258 homodimer 2 * 28000 Moorella thermoacetica

Synonyms

EC Number Synonyms Comment Organism
2.1.1.258 methyltetrahydrofolate, corrinoid iron–sulfur protein methyltransferase
-
 Moorella thermoacetica
2.1.1.258 MeTr
-
 Moorella thermoacetica

General Information

EC Number General Information Comment Organism
2.1.1.258 metabolism the enzyme catalyzes a key step in the Wood-Ljungdahl pathway of carbon dioxide fixation Moorella thermoacetica