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Literature summary extracted from
- A study on L-asparaginase of Nocardia levis MK-VL_113 (2012), ScientificWorldJournal, 2012, 160434.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.1 | additional information | cell disrupting agents EDTA, lysozyme, and penicillin-G enhance the release of L-asparaginase from the cell culture | Nocardia levis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.1 | L-asparagine + H2O | Nocardia levis | - |
L-aspartate + NH3 | - |
? |  |
| 3.5.1.1 | L-asparagine + H2O | Nocardia levis MK-VL_113 | - |
L-aspartate + NH3 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.1 | Nocardia levis | - |
isolated from laterite soils of Guntur region, India | - |
| 3.5.1.1 | Nocardia levis MK-VL_113 | - |
isolated from laterite soils of Guntur region, India | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.1.1 | cell suspension culture | optimization of production of L-asparaginase by strain MK-VL_113. Optimal production at pH 7.0 and 30°C in a 72 h old culture in modified ISP-5 medium containing yeast extract, tryptone, or L-asparagine at 1-1.5% w/v as nitrogen source and 2% glycerol as carbon source, overview | Nocardia levis | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.1 | L-asparagine + H2O | - |
Nocardia levis | L-aspartate + NH3 | - |
? | |
| 3.5.1.1 | L-asparagine + H2O | - |
Nocardia levis MK-VL_113 | L-aspartate + NH3 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.1 | L-asparaginase | - |
Nocardia levis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 37 | - |
assay at | Nocardia levis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 7.5 | - |
assay at | Nocardia levis |
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