EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.257 | additional information | Methanocaldococcus jannaschii TrmY requires C55 (or possibly U55) in the tRNA to produce m1PSI54 | Methanocaldococcus jannaschii |
EC Number | Application | Comment | Organism |
---|---|---|---|
5.4.99.B25 | molecular biology | using the COG1901 recombinant enzyme from Methanocaldococcus jannaschii (Mj1640), purified enzyme Pus10 from Methanocaldococcus jannaschii and full-size tRNA transcripts or T(PSI)-arm (17-mer) fragments as substrates, the sequential pathway of m(1)PSI54 formation in Archaea is reconstituted | Methanocaldococcus jannaschii |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.257 | gene trmY or HVO_1989 | Haloferax volcanii |
2.1.1.257 | gene VNG1980C, expression of the gene encoding COG1901 from Halobacterium sp. strain NRC-1 in Haloferax volcanii strain DHVO_1989 complements the m1PSI minus, enzyme-deficient phenotype of the mutant strain | Halobacterium sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.257 | additional information | expression of the gene VNG1980C encoding COG1901 from Halobacterium sp. strain NRC-1 in Haloferax volcanii strain DHVO_1989 complements the m1PSI minus, enzyme-deficient phenotype of the mutant strain | Haloferax volcanii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.257 | S-adenosyl-L-homocysteine | - |
Halobacterium sp. | |
2.1.1.257 | S-adenosyl-L-homocysteine | - |
Haloferax volcanii | |
2.1.1.257 | S-adenosyl-L-homocysteine | - |
Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.257 | S-adenosyl-L-methionine + pseudouridine54 in tRNA | Haloferax volcanii | - |
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
2.1.1.257 | S-adenosyl-L-methionine + pseudouridine54 in tRNA | Halobacterium sp. | - |
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
2.1.1.257 | S-adenosyl-L-methionine + pseudouridine54 in tRNA | Methanocaldococcus jannaschii | - |
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
2.1.1.257 | S-adenosyl-L-methionine + pseudouridine54 in tRNA | Halobacterium sp. NRC-1 | - |
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
5.4.99.B25 | tRNA uridine54 | Methanocaldococcus jannaschii | - |
tRNA pseudouridine54 | - |
? | |
5.4.99.B25 | tRNA uridine54 | Methanocaldococcus jannaschii DSM 2661 | - |
tRNA pseudouridine54 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.257 | Halobacterium sp. | Q9HNR6 | gene VNG1980C | - |
2.1.1.257 | Halobacterium sp. NRC-1 | Q9HNR6 | gene VNG1980C | - |
2.1.1.257 | Haloferax volcanii | D4GTL8 | gene HVO_1989 or trmY | - |
2.1.1.257 | Methanocaldococcus jannaschii | Q59034 | gene Mj1640 | - |
5.4.99.B25 | Methanocaldococcus jannaschii | Q60346 | - |
- |
5.4.99.B25 | Methanocaldococcus jannaschii DSM 2661 | Q60346 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.257 | additional information | enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview | Haloferax volcanii | ? | - |
? | |
2.1.1.257 | additional information | enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview | Halobacterium sp. | ? | - |
? | |
2.1.1.257 | additional information | enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview | Methanocaldococcus jannaschii | ? | - |
? | |
2.1.1.257 | additional information | enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview | Halobacterium sp. NRC-1 | ? | - |
? | |
2.1.1.257 | S-adenosyl-L-methionine + pseudouridine54 in elongator tRNAMet | - |
Haloferax volcanii | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in elongator tRNAMet | - |
? | |
2.1.1.257 | S-adenosyl-L-methionine + pseudouridine54 in tRNA | - |
Haloferax volcanii | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
2.1.1.257 | S-adenosyl-L-methionine + pseudouridine54 in tRNA | - |
Halobacterium sp. | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
2.1.1.257 | S-adenosyl-L-methionine + pseudouridine54 in tRNA | - |
Methanocaldococcus jannaschii | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
2.1.1.257 | S-adenosyl-L-methionine + pseudouridine54 in tRNA | methylation of pseudouridine PSI at position 54 of tRNA, in vitro and in vivo reaction, producing m1PSI, in vitro pseudouridylated tRNATrp is used as a substrate | Methanocaldococcus jannaschii | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
2.1.1.257 | S-adenosyl-L-methionine + pseudouridine54 in tRNA | methylation of pseudouridine PSI at position 54 of tRNA, producing m1PSI, in vitro pseudouridylated tRNATrp is used as a substrate | Haloferax volcanii | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
2.1.1.257 | S-adenosyl-L-methionine + pseudouridine54 in tRNA | methylation of pseudouridine PSI at position 54 of tRNA, producing m1PSI, in vitro pseudouridylated tRNATrp is used as a substrate | Halobacterium sp. | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
2.1.1.257 | S-adenosyl-L-methionine + pseudouridine54 in tRNA | - |
Halobacterium sp. NRC-1 | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
2.1.1.257 | S-adenosyl-L-methionine + pseudouridine54 in tRNA | methylation of pseudouridine PSI at position 54 of tRNA, producing m1PSI, in vitro pseudouridylated tRNATrp is used as a substrate | Halobacterium sp. NRC-1 | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
2.1.1.257 | S-adenosyl-L-methionine + pseudouridine54 in tRNATrp | - |
Haloferax volcanii | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNATrp | - |
? | |
5.4.99.B25 | tRNA uridine54 | - |
Methanocaldococcus jannaschii | tRNA pseudouridine54 | - |
? | |
5.4.99.B25 | tRNA uridine54 | - |
Methanocaldococcus jannaschii DSM 2661 | tRNA pseudouridine54 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.257 | COG1901 | - |
Haloferax volcanii |
2.1.1.257 | COG1901 | - |
Halobacterium sp. |
2.1.1.257 | COG1901 | - |
Methanocaldococcus jannaschii |
2.1.1.257 | DUF358 | - |
Haloferax volcanii |
2.1.1.257 | DUF358 | - |
Halobacterium sp. |
2.1.1.257 | DUF358 | - |
Methanocaldococcus jannaschii |
2.1.1.257 | SAM-dependent N1-pseudouridine methyltransferase | - |
Methanocaldococcus jannaschii |
2.1.1.257 | TrmY protein | - |
Haloferax volcanii |
2.1.1.257 | TrmY protein | - |
Halobacterium sp. |
2.1.1.257 | TrmY protein | - |
Methanocaldococcus jannaschii |
5.4.99.B25 | Pus10 | - |
Methanocaldococcus jannaschii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.1.257 | evolution | m1PSI formation in archaeal tRNAs, overview. The enzyme belongs to Cluster of Orthologous Group, COG, 1901 | Haloferax volcanii |
2.1.1.257 | evolution | m1PSI formation in archaeal tRNAs, overview. The enzyme belongs to Cluster of Orthologous Group, COG, 1901 | Halobacterium sp. |
2.1.1.257 | evolution | m1PSI formation in archaeal tRNAs, overview. The enzyme belongs to Cluster of Orthologous Group, COG, 1901 | Methanocaldococcus jannaschii |
2.1.1.257 | malfunction | deletion of trmY leads to the absence of m1PSI54 in Haloferax volcanii tRNA | Haloferax volcanii |
2.1.1.257 | metabolism | Pus10-mediated PSI54 (and PSI55) formation does not require the presence of TrmY activity, overview | Haloferax volcanii |
2.1.1.257 | metabolism | Pus10-mediated PSI54 (and PSI55) formation does not require the presence of TrmY activity, overview | Methanocaldococcus jannaschii |
2.1.1.257 | additional information | Methanocaldococcus jannaschii TrmY requires C55 (or possibly U55) in the tRNA to produce m1PSI54 | Methanocaldococcus jannaschii |
2.1.1.257 | physiological function | trmY is not essential in Haloferax volcanii | Haloferax volcanii |