EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.176 | gene TTHA0851 | Thermus thermophilus |
2.1.1.178 | gene TTHA1387 | Thermus thermophilus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.1.1.178 | RsmF in a complex with cofactor S-adenosyl-methionine, three crystal structures, X-ray diffrcation structure determination and analysis at 1.3 A resolution | Thermus thermophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.176 | additional information | construction of a null mutant strain in which TTHA0851 is inactivated by homologous recombination and insertion of a heat stable kanamycin-resistance gene | Thermus thermophilus |
2.1.1.178 | additional information | construction of a null mutant strain in which TTHA1387 is inactivated by homologous recombination and insertion of a heat stable kanamycin-resistance gene | Thermus thermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.176 | S-adenosyl-L-methionine + cytosine967 in 16S rRNA | Thermus thermophilus | - |
S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA | - |
? | |
2.1.1.176 | S-adenosyl-L-methionine + cytosine967 in 16S rRNA | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA | - |
? | |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1400 in 16S rRNA | Thermus thermophilus | - |
S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA | - |
? | |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1400 in 16S rRNA | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA | - |
? | |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1404 in 16S rRNA | Thermus thermophilus | - |
S-adenosyl-L-homocysteine + 5-methylcytosine1404 in 16S rRNA | - |
? | |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1407 in 16S rRNA | Thermus thermophilus | - |
S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA | - |
? | |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1407 in 16S rRNA | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.176 | Thermus thermophilus | - |
gene TTHA0851 or rsmB | - |
2.1.1.176 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
gene TTHA0851 or rsmB | - |
2.1.1.178 | Thermus thermophilus | Q5SJI2 | gene TTHA1387 or rsmF | - |
2.1.1.178 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q5SJI2 | gene TTHA1387 or rsmF | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.176 | additional information | substrate recognition mechanism, overview | Thermus thermophilus | ? | - |
? | |
2.1.1.176 | additional information | substrate recognition mechanism, overview | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
2.1.1.176 | S-adenosyl-L-methionine + cytosine967 in 16S rRNA | - |
Thermus thermophilus | S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA | - |
? | |
2.1.1.176 | S-adenosyl-L-methionine + cytosine967 in 16S rRNA | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA | - |
? | |
2.1.1.178 | additional information | substrate recognition mechanism, overview | Thermus thermophilus | ? | - |
? | |
2.1.1.178 | additional information | substrate recognition mechanism, overview | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1400 in 16S rRNA | - |
Thermus thermophilus | S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA | - |
? | |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1400 in 16S rRNA | RsmF completely methylates C1400 when either 16S rRNA or 30S subunits are used as a substrate | Thermus thermophilus | S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA | - |
? | |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1400 in 16S rRNA | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA | - |
? | |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1400 in 16S rRNA | RsmF completely methylates C1400 when either 16S rRNA or 30S subunits are used as a substrate | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA | - |
? | |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1404 in 16S rRNA | - |
Thermus thermophilus | S-adenosyl-L-homocysteine + 5-methylcytosine1404 in 16S rRNA | - |
? | |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1404 in 16S rRNA | RsmF methylates C1404 to about 35% with 16S rRNA and completely with 30S subunits | Thermus thermophilus | S-adenosyl-L-homocysteine + 5-methylcytosine1404 in 16S rRNA | - |
? | |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1407 in 16S rRNA | - |
Thermus thermophilus | S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA | - |
? | |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1407 in 16S rRNA | RsmF produces only trace amounts of methylation of C1407 with 16S rRNA and about 75% with 30S subunits | Thermus thermophilus | S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA | - |
? | |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1407 in 16S rRNA | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA | - |
? | |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1407 in 16S rRNA | RsmF produces only trace amounts of methylation of C1407 with 16S rRNA and about 75% with 30S subunits | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.176 | More | comparison of Thermus thermophilus RsmB and RsmF protein sequences, secondary and tertiary structure, differences in substrate binding, overview | Thermus thermophilus |
2.1.1.178 | More | comparison of Thermus thermophilus RsmB and RsmF protein sequences, secondary and tertiary structure, differences in substrate binding, overview | Thermus thermophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.176 | 16S rRNA methyltransferase | - |
Thermus thermophilus |
2.1.1.176 | fmu | - |
Thermus thermophilus |
2.1.1.176 | rsmB | - |
Thermus thermophilus |
2.1.1.178 | 16S rRNA methyltransferase | - |
Thermus thermophilus |
2.1.1.178 | RsmF | - |
Thermus thermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.178 | 60 | 70 | no difference in the relative growth proportions of the wild-type and rsmF mutant at 70°C. However, at 60°C, the rsmF null mutant constitutes only around 5% of the population, and at 80°C the rsmF null mutant is unable to grow at all | Thermus thermophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.176 | S-adenosyl-L-methionine | - |
Thermus thermophilus | |
2.1.1.178 | S-adenosyl-L-methionine | - |
Thermus thermophilus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.1.176 | metabolism | two methyltransferases, RsmB and RsmF, are responsible for all four 5-methylcytidine modifications in 16S rRNA of Thermus thermophilus, overview. RsmB produces m5C967, while RsmF methylates C1400, C1404, and C1407 in a 30S subunit substrate, but only C1400 and C1404 when naked 16S rRNA is the substrate | Thermus thermophilus |
2.1.1.178 | metabolism | two methyltransferases, RsmB and RsmF, are responsible for all four 5-methylcytidine modifications in 16S rRNA of Thermus thermophilus, overview. RsmB produces m5C967, while RsmF methylates C1400, C1404, and C1407 in a 30S subunit substrate, but only C1400 and C1404 when naked 16S rRNA is the substrate. C1400 and C1404 are sufficiently buried in the mature ribosome structure so require extensive unfolding of the rRNA to be accessible to RsmF | Thermus thermophilus |