Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Santos, C.; Tonoli, C.; Trindade, D.; Betzel, C.; Takata, H.; Kuriki, T.; Kanai, T.; Imanaka, T.; Arni, R.; Murakami, M.
    Structural basis for branching-enzyme activity of glycoside hydrolase family 57: Structure and stability studies of a novel branching enzyme from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 (2011), Proteins, 79, 547-557.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.18 in the native state and in complex with glucose and substrate mimetics, to 2.4 A, 2.9 A, and 1.9 A resolution, respectively. The structure encompasses a distorted (beta/alpha)7-barrel juxtaposed to a C-terminal alpha-helical domain, which also participates in the formation of the active-site cleft. The active site comprises two acidic catalytic residues, Glu183 and Asp354, the polarizer His10, aromatic gate-keepers Trp28, Trp270, Trp407, and Trp416 and the residue Tyr233, which is fully conserved among GH13- and GH57-type branching enzymes Thermococcus kodakarensis

General Stability

EC Number General Stability Organism
2.4.1.18 stable protein even at high concentrations of chaotropic agents Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.18 Thermococcus kodakarensis Q5JDJ7 member of family GH57
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.4.1.18 unfolding equilibrium is a two-state process with no intermediates Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.18 additional information loop 220-245, named as catalytic loop, acts as a lid retaining the intermediate reaction product for subsequent transfer to a new a-1,6 position. The active site comprises two acidic catalytic residues, Glu183 and Asp354, the polarizer His10, aromatic gate-keepers Trp28, Trp270, Trp407, and Trp416 and the residue Tyr233 Thermococcus kodakarensis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.18 TK1436
-
 Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.18 additional information
-
 high thermal tolerance even at boiling temperatures Thermococcus kodakarensis