EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.148 | NADPH | 0.1 mM NADPH saturates the enzymatic reaction | Helicobacter pylori |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.148 | expressed in Escherichia coli M15 cells | Helicobacter pylori |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.1.1.148 | hanging drop vapor diffusion method, using 0.2 M sodium chloride, 0.1 M MES, pH 6.0, and 20% (w/v) PEG2000 monomethyl ether | Helicobacter pylori |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.45 | 10-methylfolate | - |
Helicobacter pylori | |
2.1.1.45 | 5-fluoro-2'-deoxyuridine monophosphate | - |
Helicobacter pylori | |
2.1.1.45 | 8-azapurinone deoxyribonucleoside 5'-phosphate | - |
Helicobacter pylori | |
2.1.1.45 | N-(4-{[(2-amino-4-oxo-1,4-dihydroquinazolin-6-yl)methyl](prop-2-ynoyl)amino}benzoyl)-L-glutamic acid | - |
Helicobacter pylori | |
2.1.1.148 | NADPH | excess NADPH decreases enzyme activity | Helicobacter pylori |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.148 | 0.0153 | - |
dUMP | in 50 mM Tris-HCl pH 8.0, 10 mM MgCl2, 2 mM NADPH, 0.5 mM FAD, at 22°C | Helicobacter pylori | |
2.1.1.148 | 0.35 | - |
5,10-methylenetetrahydrofolate | in 50 mM Tris-HCl pH 8.0, 10 mM MgCl2, 2 mM NADPH, 0.5 mM FAD, at 22°C | Helicobacter pylori |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.148 | 25000 | - |
4 * 25000, SDS-PAGE | Helicobacter pylori |
2.1.1.148 | 25020 | - |
4 * 25020, calculated from amino acid sequence | Helicobacter pylori |
2.1.1.148 | 25066 | - |
4 * 25066, MALDI TOF mass spectrometry | Helicobacter pylori |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.45 | Helicobacter pylori | - |
- |
- |
2.1.1.45 | Helicobacter pylori SS1 | - |
- |
- |
2.1.1.148 | Helicobacter pylori | Q5UVJ4 | - |
- |
2.1.1.148 | Helicobacter pylori SS1 | Q5UVJ4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.148 | nickel chelating column chromatography, GSH-Sepharose column chromatography, and Superdex 200 gel filtration | Helicobacter pylori |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Helicobacter pylori | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Helicobacter pylori SS1 | dihydrofolate + dTMP | - |
? | |
2.1.1.148 | 5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Helicobacter pylori | dTMP + tetrahydrofolate + FAD | - |
? | |
2.1.1.148 | 5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Helicobacter pylori SS1 | dTMP + tetrahydrofolate + FAD | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.148 | homotetramer | 4 * 25000, SDS-PAGE | Helicobacter pylori |
2.1.1.148 | homotetramer | 4 * 25020, calculated from amino acid sequence | Helicobacter pylori |
2.1.1.148 | homotetramer | 4 * 25066, MALDI TOF mass spectrometry | Helicobacter pylori |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.45 | ThyA | - |
Helicobacter pylori |
2.1.1.45 | thymidylate synthase A | - |
Helicobacter pylori |
2.1.1.148 | thymidylate synthase X | - |
Helicobacter pylori |
2.1.1.148 | ThyX | - |
Helicobacter pylori |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.148 | 8.5 | - |
- |
Helicobacter pylori |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.148 | 6.5 | 10.5 | - |
Helicobacter pylori |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.148 | FAD | the enzyme is bound to 4 FAD molecules | Helicobacter pylori |