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Literature summary extracted from

  • Solovjeva, O.N.; Sevostyanova, I.A.; Yurshev, V.A.; Selivanov, V.A.; Kochetov, G.A.
    Effects of free Ca2+ on kinetic characteristics of holotransketolase (2012), Protein J., 31, 137-140.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.1 0.007
-
D-ribose 5-phosphate and second value 0.698 mM, pH 7.6, presence of 0.1 mM Ca2+, temperature not specified in the publication Saccharomyces cerevisiae
2.2.1.1 0.025
-
D-xylulose 5-phosphate and second value 0.773 mM, pH 7.6, presence of 0.1 mM Ca2+, temperature not specified in the publication Saccharomyces cerevisiae
2.2.1.1 0.075
-
D-xylulose 5-phosphate pH 7.6, temperature not specified in the publication Saccharomyces cerevisiae
2.2.1.1 0.159
-
D-ribose 5-phosphate pH 7.6, temperature not specified in the publication Saccharomyces cerevisiae
2.2.1.1 0.698
-
D-ribose 5-phosphate and first value 0.007 mM, pH 7.6, presence of 0.1 mM Ca2+, temperature not specified in the publication Saccharomyces cerevisiae
2.2.1.1 0.773
-
D-xylulose 5-phosphate and first value 0.025 mM, pH 7.6, presence of 0.1 mM Ca2+, temperature not specified in the publication Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.1 Ca2+ in the presence of free Ca2+, the equivalence of the two active centers of the enzyme is lost. Negative cooperativity is induced on binding of either xylulose 5-phosphate or ribose 5-phosphate, whereupon the catalytic conversion of the bound substrates causes the interaction between the centers to become positively cooperative. Enzyme total activity increases in presence of Ca2+ Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.1
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate
-
Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
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