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Literature summary extracted from

  • Roy, I.; Smith, O.; Clouthier, C.M.; Keillor, J.W.
    Expression, purification and kinetic characterisation of human tissue transglutaminase (2013), Protein Expr. Purif., 87, 41-46.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.13 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.13 acetonitrile 48% residual activity at 5% (v/v) Homo sapiens
2.3.2.13 acrylonitrile 68 and 48% residual activity at 2.5 and 5.0 mM, respectively Homo sapiens
2.3.2.13 CP30a reversible inhibitor Homo sapiens
2.3.2.13 CP4d reversible inhibitor Homo sapiens
2.3.2.13 DMF 90% residual activity at 2.5 and 5% (v/v) Homo sapiens
2.3.2.13 NC-I052 irreversible inhibitor Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.13 0.011
-
 N-acetyl-PNPQLPF apparent value, 0.05 mM EDTA, 3.3 mM CaCl2, in 0.11 M MOPS at pH 7.0 and 25°C Homo sapiens
2.3.2.13 0.014
-
 Ac-PNPQLPF-OH at pH 7.0 and 25°C Homo sapiens
2.3.2.13 0.014
-
 N-Cbz-L-glutaminyl(gamma-4-nitrophenylester)glycine 0.05 mM EDTA, 3.3 mM CaCl2, in 0.11 M MOPS at pH 7.0 and 25°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.13 Ca2+ dependent on Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.13 77600
-
 x * 77600, untagged enzyme, estimated from SDS-PAGE Homo sapiens
2.3.2.13 77600
-
 x * 77600, calculated from SDS-PAGE Homo sapiens
2.3.2.13 104000
-
 x * 104000, GST and PreScission Protease-tagged enzyme, estimated from SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.13 N-acetyl-PNPQLPF + alkylamine Homo sapiens
-
 ?
-
 ?
2.3.2.13 N-Cbz-L-glutaminyl(gamma-4-nitrophenylester)glycine + alkylamine Homo sapiens
-
 ?
-
 ?
2.3.2.13 [protein]-L-glutamine + alkylamine Homo sapiens
-
 [protein]-N5-alkyl-L-glutamine + NH3
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.3.2.13 dimethylformamide about 90% activity at 2.5 and 5.0 mM dimethylformamide Homo sapiens
2.3.2.13 DMSO 100% activity in the presence of 2.5 and 5.0 mM DMSO Homo sapiens
2.3.2.13 additional information the enzyme is not affected by DMSO Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Homo sapiens P21980
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13 glutathione Sepharose 4B resin chromatography Homo sapiens
2.3.2.13 glutathione Sepharose column chromatography Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.13 0.01
-
 recombinant enzyme from lysate supernatant, at pH 7.0 and 25°C Homo sapiens
2.3.2.13 0.01
-
 unpurified enzyme, with N-Cbz-Glu(gamma-4-nitrophenylester)Gly as substrate, at pH 7.0 and 25°C Homo sapiens
2.3.2.13 0.33
-
 purified recombinant enzyme, at pH 7.0 and 25°C Homo sapiens
2.3.2.13 0.33
-
 purified enzyme, with N-Cbz-Glu(gamma-4-nitrophenylester)Gly as substrate, at pH 7.0 and 25°C Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.3.2.13 -80C, purified enzyme, 0.05 mM EDTA, 3.3 mM CaCl2, in 0.11 M MOPS at pH 7.0, several months, no loss of activity Homo sapiens
2.3.2.13 4°C, lyophilised purified enzyme, 1 week, 80% loss of activity Homo sapiens
2.3.2.13 4°C, purified enzyme, 0.05 mM EDTA, 3.3 mM CaCl2, in 0.11 M MOPS at pH 7.0, 1 week, 10% loss of activity Homo sapiens
2.3.2.13 4°C, purified enzyme, 0.05 mM EDTA, 3.3 mM CaCl2, in 0.11 M MOPS at pH 7.0, 2 weeks, 25% loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 Ac-PNPQLPF-OH + alkylamine
-
 Homo sapiens ?
-
 ?
2.3.2.13 N-acetyl-PNPQLPF + alkylamine
-
 Homo sapiens ?
-
 ?
2.3.2.13 N-Cbz-Glu(gamma-4-nitrophenylester)Gly + alkylamine
-
 Homo sapiens 4-nitrophenol + ?
-
 ?
2.3.2.13 N-Cbz-L-glutaminyl(gamma-4-nitrophenylester)glycine + alkylamine
-
 Homo sapiens ?
-
 ?
2.3.2.13 [protein]-L-glutamine + alkylamine
-
 Homo sapiens [protein]-N5-alkyl-L-glutamine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.13 ? x * 104000, GST and PreScission Protease-tagged enzyme, estimated from SDS-PAGE Homo sapiens
2.3.2.13 ? x * 77600, untagged enzyme, estimated from SDS-PAGE Homo sapiens
2.3.2.13 ? x * 77600, calculated from SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.3.2.13 TG2
-
 Homo sapiens
2.3.2.13 TGase
-
 Homo sapiens
2.3.2.13 tissue transglutaminase
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.2.13 0.001
-
 CP4d at pH 7.0 and 25°C Homo sapiens
2.3.2.13 0.001
-
 CP4d 0.05 mM EDTA, 3.3 mM CaCl2, in 0.11 M MOPS at pH 7.0 and 25°C Homo sapiens
2.3.2.13 0.004
-
 CP30a at pH 7.0 and 25°C Homo sapiens
2.3.2.13 0.004
-
 CP30a 0.05 mM EDTA, 3.3 mM CaCl2, in 0.11 M MOPS at pH 7.0 and 25°C Homo sapiens
2.3.2.13 0.019
-
 NC-I052 at pH 7.0 and 25°C Homo sapiens
2.3.2.13 0.019
-
 NC-I052 0.05 mM EDTA, 3.3 mM CaCl2, in 0.11 M MOPS at pH 7.0 and 25°C Homo sapiens