Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhu, X.; Gu, X.; Zhang, S.; Liu, Y.; Huang, Z.; Tan, X.
    Efficient expression and purification of methyltransferase in acetyl-coenzyme a synthesis pathway of the human pathogen Clostridium difficile (2011), Protein Expr. Purif., 78, 86-93.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.258 expressed in Escherichia coli Rosetta (DE3) pLysS cells Clostridioides difficile

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.258 0.058
-
 hydroxycobalamin recombinant enzyme, in 0.1 M MES, at pH 5.1 and 25°C Clostridioides difficile

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.258 30000
-
 2 * 30000, SDS-PAGE Clostridioides difficile
2.1.1.258 31000
-
 2 * 31000, calculated from amino acid sequence Clostridioides difficile

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.258 Clostridioides difficile
-
-
-
2.1.1.258 Clostridioides difficile 630
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.258 amylose resin column chromatography, Ni-NTA column chromatography, Sephadex-G25 gel filtration, and Superdex 75 gel filtration Clostridioides difficile

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.258 hydroxycobalamin + 5-methyltetrahydrofolate
-
 Clostridioides difficile methylcobalamin + tetrahydrofolate
-
 ?
2.1.1.258 hydroxycobalamin + 5-methyltetrahydrofolate
-
 Clostridioides difficile 630 methylcobalamin + tetrahydrofolate
-
 ?
2.1.1.258 [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrofolate
-
 Clostridioides difficile [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrofolate
-
 ?
2.1.1.258 [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrofolate
-
 Clostridioides difficile 630 [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrofolate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.258 homodimer 2 * 30000, SDS-PAGE Clostridioides difficile
2.1.1.258 homodimer 2 * 31000, calculated from amino acid sequence Clostridioides difficile

Synonyms

EC Number Synonyms Comment Organism
2.1.1.258 methyltetrahydrofolate- and corrinoid-dependent methyltransferase
-
 Clostridioides difficile
2.1.1.258 MeTr
-
 Clostridioides difficile

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.258 16
-
 the recombinant enzyme is not stable at 16°C or higher temperature Clostridioides difficile

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.258 77.6
-
 hydroxycobalamin recombinant enzyme, in 0.1 M MES, at pH 5.1 and 25°C Clostridioides difficile

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.258 1330
-
 hydroxycobalamin recombinant enzyme, in 0.1 M MES, at pH 5.1 and 25°C Clostridioides difficile