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Literature summary extracted from

  • Hamdane, D.; Skouloubris, S.; Myllykallio, H.; Golinelli-Pimpaneau, B.
    Expression and purification of untagged and histidine-tagged folate-dependent tRNA:m5U54 methyltransferase from Bacillus subtilis (2010), Protein Expr. Purif., 73, 83-89.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.74 untagged N-terminus and C-terminus (His)6-tagged TrmFO from Bacillus subtilis is expressed in Escherichia coli. The tag does not significantly alter the expression level, flavin content, activity and secondary structure of the protein Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.74 R312G mutant isolated after cloning is found to be defective in tRNA methylation, with an activity corresponding only to 40% that of the wild-type enzyme Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.74 33600
-
untagged protein Bacillus subtilis
2.1.1.74 47900
-
His-tagged fusion protein Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.74 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.74 purified to homogeneity by metal affinity or ion exchange and heparin affinity, respectively, followed by gel filtration Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + NADH
-
Bacillus subtilis tetrahydrofolate + tRNA TpsiC + NAD+
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.74 TRMFO
-
Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.74 37
-
assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.74 7.5
-
assay at Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.74 NADH is used in the assay as electron donor Bacillus subtilis