EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.74 | untagged N-terminus and C-terminus (His)6-tagged TrmFO from Bacillus subtilis is expressed in Escherichia coli. The tag does not significantly alter the expression level, flavin content, activity and secondary structure of the protein | Bacillus subtilis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.74 | R312G | mutant isolated after cloning is found to be defective in tRNA methylation, with an activity corresponding only to 40% that of the wild-type enzyme | Bacillus subtilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.74 | 33600 | - |
untagged protein | Bacillus subtilis |
2.1.1.74 | 47900 | - |
His-tagged fusion protein | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.74 | Bacillus subtilis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.74 | purified to homogeneity by metal affinity or ion exchange and heparin affinity, respectively, followed by gel filtration | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.74 | 5,10-methylenetetrahydrofolate + tRNA UpsiC + NADH | - |
Bacillus subtilis | tetrahydrofolate + tRNA TpsiC + NAD+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.74 | TRMFO | - |
Bacillus subtilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.74 | 37 | - |
assay at | Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.74 | 7.5 | - |
assay at | Bacillus subtilis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.74 | NADH | is used in the assay as electron donor | Bacillus subtilis |