EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
7.6.2.1 | F511L | the mutant has a specific activity that is 35% that of wild type Drs2 | Saccharomyces cerevisiae |
7.6.2.1 | F511Y | the mutant retains wild type activity, the substitution in Drs2 specifically abrogates phosphatidylserine recognition by this flippase causing phosphatidylserine exposure on the outer leaflet of the plasma membrane without disrupting phospatidylethanolamine asymmetry | Saccharomyces cerevisiae |
7.6.2.1 | Y618F | acquisition of the phosphatidylserine substrate maps to a Tyr618Phe substitution in transmembrane segment 4 of Dnf1. The rate of 7-nitrobenz-2oxa-1,3-diazol-4-yl phospholipid uptake by Dnf1 Y618F is comparable to wild type Dnf1 | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.6.2.1 | Saccharomyces cerevisiae | P32660 | - |
- |
7.6.2.1 | Saccharomyces cerevisiae | P39524 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.6.2.1 | ATP + H2O + phosphatidylcholine/in | - |
Saccharomyces cerevisiae | ADP + phosphate + phosphatidylcholine/out | - |
? | |
7.6.2.1 | ATP + H2O + phosphatidylcholine/in | preferred substrate of Dnf1 | Saccharomyces cerevisiae | ADP + phosphate + phosphatidylcholine/out | - |
? | |
7.6.2.1 | ATP + H2O + phosphatidylserine/in | - |
Saccharomyces cerevisiae | ADP + phosphate + phosphatidylserine/out | - |
? | |
7.6.2.1 | ATP + H2O + phosphatidylserine/in | preferred substrate of Drs2 | Saccharomyces cerevisiae | ADP + phosphate + phosphatidylserine/out | - |
? | |
7.6.2.1 | additional information | wild type Dnf1 cannot translocate NBD-SM or NBD-PA | Saccharomyces cerevisiae | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.6.2.1 | Dnf1 | - |
Saccharomyces cerevisiae |
7.6.2.1 | Drs2 | - |
Saccharomyces cerevisiae |
7.6.2.1 | P4-ATPase | - |
Saccharomyces cerevisiae |
7.6.2.1 | phospholipid flippase | - |
Saccharomyces cerevisiae |
7.6.2.1 | type IV P-type ATPase | - |
Saccharomyces cerevisiae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
7.6.2.1 | physiological function | type IV P-type ATPases catalyzes translocation of phospholipid across a membrane to establish an asymmetric bilayer structure with phosphatidylserine and phosphatidylethanolamine restricted to the cytosolic leaflet | Saccharomyces cerevisiae |