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Literature summary extracted from

  • Zhang, Y.; Yang, S.; Liu, M.; Song, C.; Wu, N.; Ling, P.; Chu, E.; Lin, X.
    Interaction between thymidylate synthase and its cognate mRNA in zebrafish embryos (2010), PLoS ONE, 5, e10618.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.1.1.45 treatment of zebrafish embryos with 5-fluorouracil increases the enzyme stability significantly, the half life of enzyme protein is about 2.7 times longer than in untreated embryos Danio rerio

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Danio rerio
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.45 embryo
-
Danio rerio
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Danio rerio dihydrofolate + dTMP
-
?
2.1.1.45 additional information the enzyme is specifically bound with its cognate mRNA in vitro and in vivo Danio rerio ?
-
?

Expression

EC Number Organism Comment Expression
2.1.1.45 Danio rerio the expression of thymidylate synthase is increased by about 6fold when embryos are treated with 0.001 mM 5-fluorouracil and there is a greater than 10fold increase in the enzyme protein level after treatment with 0.0004 mM ZD1694 up

General Information

EC Number General Information Comment Organism
2.1.1.45 physiological function the enzyme is indispensable for DNA replication in actively dividing cells Danio rerio