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Literature summary extracted from

  • Landmann, C.; Huecherig, S.; Fink, B.; Hoffmann, T.; Dittlein, D.; Coiner, H.A.; Schwab, W.
    Substrate promiscuity of a rosmarinic acid synthase from lavender (Lavandula angustifolia L.) (2011), Planta, 234, 305-320.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.110 AAT1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree of BAHD acyltransferases, expression of GST-tagged AAT1 in Escherichia coli strain Rosetta(DE3)pLysS Lavandula angustifolia
2.3.1.140 AAT1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree of BAHD acyltransferases, expression of GST-tagged AAT1 in Escherichia coli strain Rosetta(DE3)pLysS Lavandula angustifolia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.110 0.005
-
 tyramine with caffeoyl-CoA, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
2.3.1.110 0.018
-
 tyramine with cinnamoyl-CoA, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
2.3.1.110 0.036
-
 tyramine with 4-coumaroyl-CoA, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
2.3.1.110 0.057
-
 4-coumaroyl-CoA with tyramine, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
2.3.1.110 0.112
-
 phenethylamine with 4-coumaroyl-CoA, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
2.3.1.110 0.157
-
 4-coumaroyl-CoA with tryptamine, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
2.3.1.110 0.192
-
 feruloyl-CoA with tyramine, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
2.3.1.110 0.243
-
 tyramine with feruloyl-CoA, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
2.3.1.110 0.3
-
 4-coumaroyl-CoA with phenethylamine, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
2.3.1.110 0.326
-
 caffeoyl-CoA with tyramine, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
2.3.1.110 0.49
-
 cinnamoyl-CoA with tyramine, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
2.3.1.140 0.014
-
 4-coumaroyl-CoA with 3-(4-hydroxyphenyl)lactate, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
2.3.1.140 0.021
-
 3-(4-hydroxyphenyl)lactate with 4-coumaroyl-CoA, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
2.3.1.140 0.022
-
 feruloyl-CoA with 3-(4-hydroxyphenyl)lactate, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
2.3.1.140 0.025
-
 cinnamoyl-CoA with 3-(4-hydroxyphenyl)lactate, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
2.3.1.140 0.049
-
 3-(4-hydroxyphenyl)lactate with caffeoyl-CoA, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
2.3.1.140 0.05
-
 caffeoyl-CoA with 3-(4-hydroxyphenyl)lactate, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
2.3.1.140 0.083
-
 4-coumaroyl-CoA with shikimate, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
2.3.1.140 0.652
-
 3-(4-hydroxyphenyl)lactate with cinnamoyl-CoA, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
2.3.1.140 0.672
-
 3-(4-hydroxyphenyl)lactate with feruloyl-CoA, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.110 cytosol
-
 Lavandula angustifolia 5829
-
2.3.1.140 cytosol
-
 Lavandula angustifolia 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.110 50600
-
 1 * 50600, about, LAAT1, sequence determination Lavandula angustifolia
2.3.1.140 50600
-
 1 * 50600, about, LAAT1, sequence determination Lavandula angustifolia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.110 additional information Lavandula angustifolia recombinant LaAAT1 exhibits a broad substrate tolerance accepting (hydroxy)cinnamoyl-CoAs as acyl donors and not only tyramine, tryptamine, phenylethylamine, and anthranilic acid but also shikimic acid and 4-hydroxyphenyllactic acid as acceptors, showing substrate promiscuity ?
-
 ?
2.3.1.140 caffeoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate Lavandula angustifolia
-
 CoA + rosmarinate
-
 ?
2.3.1.140 additional information Lavandula angustifolia recombinant LaAAT1 exhibits a broad substrate tolerance accepting (hydroxy)cinnamoyl-CoAs as acyl donors and not only tyramine, tryptamine, phenylethylamine, and anthranilic acid but also shikimic acid and 4-hydroxyphenyllactic acid as acceptors, showing substrate promiscuity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.110 Lavandula angustifolia Q0GA14
-
-
2.3.1.140 Lavandula angustifolia Q0GA14
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.110 recombinant GST-tagged AAT1 from Escherichia coli by cell disruption through freeze-thaw cycles, and glutathione affinity chromatography Lavandula angustifolia
2.3.1.140 recombinant GST-tagged AAT1 from Escherichia coli by cell disruption through freeze-thaw cycles, and glutathione affinity chromatography Lavandula angustifolia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.110 flower
-
 Lavandula angustifolia
-
2.3.1.140 flower
-
 Lavandula angustifolia
-

Storage Stability

EC Number Storage Stability Organism
2.3.1.110 4°C, purified recombinant GST-tagged AAT1, in 50 mM Tris-Cl, pH 8.0, and 10 mM reduced glutathione, 4 weeks, stable Lavandula angustifolia
2.3.1.140 4°C, purified recombinant GST-tagged AAT1, in 50 mM Tris-Cl, pH 8.0, and 10 mM reduced glutathione, 4 weeks, stable Lavandula angustifolia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.110 4-coumaroyl-CoA + phenethylamine
-
 Lavandula angustifolia CoA + N-(4-coumaroyl)phenethylamine
-
 ?
2.3.1.110 4-coumaroyl-CoA + tryptamine
-
 Lavandula angustifolia CoA + N-(4-coumaroyl)tryptamine
-
 ?
2.3.1.110 4-coumaroyl-CoA + tyramine
-
 Lavandula angustifolia CoA + N-(4-coumaroyl)tyramine
-
 ?
2.3.1.110 caffeoyl-CoA + tyramine
-
 Lavandula angustifolia CoA + N-caffeoyltyramine
-
 ?
2.3.1.110 cinnamoyl-CoA + tyramine
-
 Lavandula angustifolia CoA + N-cinnamoyltyramine
-
 ?
2.3.1.110 feruloyl-CoA + tyramine
-
 Lavandula angustifolia CoA + N-feruloyltyramine
-
 ?
2.3.1.110 additional information recombinant LaAAT1 exhibits a broad substrate tolerance accepting (hydroxy)cinnamoyl-CoAs as acyl donors and not only tyramine, tryptamine, phenylethylamine, and anthranilic acid but also shikimic acid and 4-hydroxyphenyllactic acid as acceptors, showing substrate promiscuity Lavandula angustifolia ?
-
 ?
2.3.1.110 additional information substrate specificity of recombinant AAT1, overview. No or poor activity with anthranilic acid Lavandula angustifolia ?
-
 ?
2.3.1.140 4-coumaroyl-CoA + 3-(4-hydroxyphenyl)lactate
-
 Lavandula angustifolia CoA + 2-O-(4-coumaroyl)-3-(4-hydroxyphenyl)lactate
-
 ?
2.3.1.140 4-coumaroyl-CoA + shikimate
-
 Lavandula angustifolia CoA + 5-O-(4-coumaroyl)shikimate
-
 ?
2.3.1.140 caffeoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
-
 Lavandula angustifolia CoA + rosmarinate
-
 ?
2.3.1.140 caffeoyl-CoA + 3-(4-hydroxyphenyl)lactate
-
 Lavandula angustifolia CoA + 2-O-caffeoyl-3-(4-hydroxyphenyl)lactate
-
 ?
2.3.1.140 cinnamoyl-CoA + 3-(4-hydroxyphenyl)lactate
-
 Lavandula angustifolia CoA + 2-O-cinnamoyl-3-(4-hydroxyphenyl)lactate
-
 ?
2.3.1.140 feruloyl-CoA + 3-(4-hydroxyphenyl)lactate
-
 Lavandula angustifolia CoA + 2-O-feruloyl-3-(4-hydroxyphenyl)lactate
-
 ?
2.3.1.140 additional information recombinant LaAAT1 exhibits a broad substrate tolerance accepting (hydroxy)cinnamoyl-CoAs as acyl donors and not only tyramine, tryptamine, phenylethylamine, and anthranilic acid but also shikimic acid and 4-hydroxyphenyllactic acid as acceptors, showing substrate promiscuity Lavandula angustifolia ?
-
 ?
2.3.1.140 additional information substrate specificity of recombinant AAT1, overview. No or poor activity with anthranilic acid Lavandula angustifolia ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.110 monomer 1 * 50600, about, LAAT1, sequence determination Lavandula angustifolia
2.3.1.140 monomer 1 * 50600, about, LAAT1, sequence determination Lavandula angustifolia

Synonyms

EC Number Synonyms Comment Organism
2.3.1.110 AAT1
-
 Lavandula angustifolia
2.3.1.110 AT1
-
 Lavandula angustifolia
2.3.1.140 AAT1
-
 Lavandula angustifolia
2.3.1.140 AT1
-
 Lavandula angustifolia
2.3.1.140 rosmarinic acid synthase
-
 Lavandula angustifolia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.110 30
-
 assay at Lavandula angustifolia
2.3.1.140 30
-
 assay at Lavandula angustifolia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.110 7.5
-
 assay at Lavandula angustifolia
2.3.1.140 7.5
-
 assay at Lavandula angustifolia

General Information

EC Number General Information Comment Organism
2.3.1.140 evolution rosmarinic acid synthase is a member of the plant BAHD family, it contains the BAHD family HXXXD motif (amino acids 180-184), with the catalytically active histidine and the DFGWG motif that is thought to be responsible for the steric position of the active site, but it additionally contains 25 additional amino acids in the section following the HXXXD motif, phylogenetic tree of BAHD acyltransferases, overview Lavandula angustifolia