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Literature summary extracted from

  • Teusch, M.; Forkmann, G.; Seyffert, W.
    Genetic control of UDP-glucose: anthocyanin 5-O-glucosyltransferase from flowers of Matthiola incana R.Br (1986), Planta, 168, 586-591.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.295 genotyping Matthiola incana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.295 Co2+ 32% inhibition at 1 mM Matthiola incana
2.4.1.295 Cu2+ complete inhibition at 1 mM Matthiola incana
2.4.1.295 diethyl dicarbonate 20% inhibition at 2 mM Matthiola incana
2.4.1.295 EDTA 32% inhibition at 2 mM Matthiola incana
2.4.1.295 Fe2+ 41% inhibition at 1 mM Matthiola incana
2.4.1.295 Mn2+ 10% inhibition at 1 mM Matthiola incana
2.4.1.295 additional information no inhibition by diethyl dithiocarbamate Matthiola incana
2.4.1.295 Zn2+ 50-60% inhibition at 1 mM Matthiola incana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.295 additional information no effect by Ca2+, Mg2+, and KCN Matthiola incana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.295 UDP-alpha-D-glucose + an anthocyanidin 3-O-beta-D-sambubioside Matthiola incana
-
 UDP + an anthocyanidin 5-O-beta-D-glucoside 3-O-beta-D-sambubioside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.295 Matthiola incana
-
 R.Br.
-
2.4.1.295 Matthiola incana
-
 different lines, overview
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.295 flower
-
 Matthiola incana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.295 dTDP-alpha-D-glucose + cyanidin 3-O-beta-D-sambubioside
-
 Matthiola incana dTDP + cyanidin 5-O-beta-D-glucoside 3-O-beta-D-sambubioside
-
 ?
2.4.1.295 dTDP-alpha-D-glucose + pelargonidin 3-O-beta-D-sambubioside
-
 Matthiola incana dTDP + pelargonidin 5-O-beta-D-glucoside 3-O-beta-D-sambubioside
-
 ?
2.4.1.295 additional information substrate specificity, overview. The enzyme catalyzes the transfer of the glucosyl moiety of uridine 5'-diphosphoglucose to the 5-hydroxyl group of pelargonidin and cyanidin 3-glycosides and acylated derivatives. The best substrate for 5-glucosylation is the 3-xylosylglucoside acylated with 4-coumarate, followed by the 3-xylosylglucoside and by the acylated (4-coumarate) 3-glucoside. The 3-glucosides themselves are a very poor substrates. No activity with UDP-xylose, -galactose, -glucuronic acid and -mannose, ADP-glucose, GDP-glucose, and CDP-glucose Matthiola incana ?
-
 ?
2.4.1.295 UDP-alpha-D-glucose + an anthocyanidin 3-O-beta-D-sambubioside
-
 Matthiola incana UDP + an anthocyanidin 5-O-beta-D-glucoside 3-O-beta-D-sambubioside
-
 ?
2.4.1.295 UDP-alpha-D-glucose + cyanidin 3-O-(6-O-(4-coumaroyl))-beta-D-sambubioside
-
 Matthiola incana UDP + cyanidin 5-O-beta-D-glucosyl-3-O-(6-O-(4-coumaroyl))-beta-D-sambubioside
-
 ?
2.4.1.295 UDP-alpha-D-glucose + cyanidin 3-O-beta-D-sambubioside
-
 Matthiola incana UDP + cyanidin 5-O-beta-D-glucoside 3-O-beta-D-sambubioside
-
 ?
2.4.1.295 UDP-alpha-D-glucose + pelargonidin 3-O-(4-coumaroyl)-beta-D-sambubioside
-
 Matthiola incana UDP + pelargonidin 5-O-beta-D-glucoside 3-O-beta-(4-coumaroyl)-D-sambubioside
-
 ?
2.4.1.295 UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-sambubioside
-
 Matthiola incana UDP + pelargonidin 5-O-beta-D-glucoside 3-O-beta-D-sambubioside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.295 5GT
-
 Matthiola incana
2.4.1.295 UDP-glucose: anthocyanin 5-O-glucosyltransferase
-
 Matthiola incana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.295 35
-
 assay at Matthiola incana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.295 7.5
-
-
 Matthiola incana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.295 6 8.5 activity range Matthiola incana