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Literature summary extracted from

  • Hashimoto, T.; Mitani, A.; Yamada, Y.
    Diamine oxidase from cultured roots of Hyoscyamus niger: its function in tropane alkaloid biosynthesis (1990), Plant Physiol., 93, 216-221.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.22 1,3-diaminopropane 10 mM with 2 mM N-methylputrescine as substrate, 32% relative inhibition Hyoscyamus niger
1.4.3.22 1,3-diaminopropane 10 mM with 2 mM N-methylputrescine as substrate, 70% relative inhibition Pisum sativum
1.4.3.22 1,3-diaminopropane 10 mM with 2 mM N-methylputrescine as substrate, 93% relative inhibition Sus scrofa
1.4.3.22 aminoguanidine 66% inhibition at 10 mM Hyoscyamus niger
1.4.3.22 CaCl2 1 mM, slightly inhibited,less than 24% Hyoscyamus niger
1.4.3.22 CdCl2 1 mM, strongly inhibited,more than 90% Hyoscyamus niger
1.4.3.22 CoCl2 1 mM, moderately inhibited,60-80% Hyoscyamus niger
1.4.3.22 CuCl2 1 mM, strongly inhibited,more than 90% Hyoscyamus niger
1.4.3.22 diethyldithiocarbamate 59% inhibition at 0.1 mM Hyoscyamus niger
1.4.3.22 FeSO4 1 mM, moderately inhibited,60-80% Hyoscyamus niger
1.4.3.22 HgCl2 1 mM, strongly inhibited,more than 90% Hyoscyamus niger
1.4.3.22 hydroxylamine 40% inhibition at 0.01 mM Hyoscyamus niger
1.4.3.22 KCN 1 mM, inhibits activity completely, is used to stop the enzyme reaction Hyoscyamus niger
1.4.3.22 MgCl2 1 mM, slightly inhibited,less than 24% Hyoscyamus niger
1.4.3.22 MnCl2 1 mM, slightly inhibited,less than 24% Hyoscyamus niger
1.4.3.22 additional information inhibition by the copper ligand diethyldithiocarbamate and the carbonyl reagents suggests that copper and pyrrroloquinoline are present at the active site of this enzyme; primary amines inhibit pea and pig kidney DAO activities more strongly than the Hyoscyamus niger. N-methylated amines are stronger inhibitors of the Hyoscyamus enzyme than of the others DAOs Hyoscyamus niger
1.4.3.22 additional information primary amines inhibit pea and pig kidney DAO activities more strongly than the Hyoscyamus niger. N-methylated amines are stronger inhibitors of the Hyoscyamus enzyme than of the others DAOs Pisum sativum
1.4.3.22 additional information primary amines inhibit pea and pig kidney DAO activities more strongly than the Hyoscyamus niger. N-methylated amines are stronger inhibitors of the Hyoscyamus enzyme than of the others DAOs Sus scrofa
1.4.3.22 n-butylamine 10 mM with 2 mM N-methylputrescine as substrate, 19% relative inhibition Hyoscyamus niger
1.4.3.22 n-butylamine 10 mM with 2 mM N-methylputrescine as substrate, 66% relative inhibition Pisum sativum
1.4.3.22 n-butylamine 10 mM with 2 mM N-methylputrescine as substrate, 60% relative inhibition Sus scrofa
1.4.3.22 N-methyl-1,3-diamonopropane 10 mM with 2 mM N-methylputrescine as substrate, 34% relative inhibition Hyoscyamus niger
1.4.3.22 N-methyl-1,3-diamonopropane 10 mM with 2 mM N-methylputrescine as substrate, 55% relative inhibition Pisum sativum
1.4.3.22 N-methyl-1,3-diamonopropane 10 mM with 2 mM N-methylputrescine as substrate, 61% relative inhibition Sus scrofa
1.4.3.22 N-methylbutylamine 10 mM with 2 mM N-methylputrescine as substrate, 70% relative inhibition Hyoscyamus niger
1.4.3.22 N-methylbutylamine 10 mM with 2 mM N-methylputrescine as substrate, 23% relative inhibition Pisum sativum
1.4.3.22 N-methylbutylamine 10 mM with 2 mM N-methylputrescine as substrate, 19% relative inhibition Sus scrofa
1.4.3.22 N-Methylmaleimide 59% inhibition at 1 mM Hyoscyamus niger
1.4.3.22 N-methylpropylamine 10 mM with 2 mM N-methylputrescine as substrate, 75% relative inhibition Hyoscyamus niger
1.4.3.22 N-methylpropylamine 10 mM with 2 mM N-methylputrescine as substrate, 13% relative inhibition Pisum sativum
1.4.3.22 N-methylpropylamine 10 mM with 2 mM N-methylputrescine as substrate, 22% relative inhibition Sus scrofa
1.4.3.22 n-Propylamine 10 mM with 2 mM N-methylputrescine as substrate, 28% relative inhibition Hyoscyamus niger
1.4.3.22 n-Propylamine 10 mM with 2 mM N-methylputrescine as substrate, 23% relative inhibition Pisum sativum
1.4.3.22 n-Propylamine 10 mM with 2 mM N-methylputrescine as substrate, 48% relative inhibition Sus scrofa
1.4.3.22 NiCl2 1 mM, moderately inhibited,60-80% Hyoscyamus niger
1.4.3.22 p-chloromercuribenzenesulfonic acid 91% inhibition at 1 mM Hyoscyamus niger
1.4.3.22 phenylhydrazine 69% inhibition at 0.01 mM Hyoscyamus niger
1.4.3.22 Semicarbazide 57% inhibition at 10 mM Hyoscyamus niger
1.4.3.22 ZnCl2 1 mM, slightly inhibited,less than 24% Hyoscyamus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.22 0.26
-
 cadaverine pH 7.9, 30°C, lowest KM-value compared with N-putrescine or cadaverine as substrates, Vmax: 96.2 pkat Pisum sativum
1.4.3.22 0.3
-
 cadaverine pH 7.9, 30°C, higher KM-value compared with N-putrescine or cadaverine as substrates, Vmax: 20.5 pkat Sus scrofa
1.4.3.22 0.31
-
 1,4-diaminobutane pH 7.9, 30°C, higher KM-value compared with N-methylputrescine or cadaverine as substrates, Vmax: 21.9 pkat Sus scrofa
1.4.3.22 0.32
-
 1,4-diaminobutane pH 7.9, 30°C, lower KM-value compared with N-methylputrescine and higher compared to cadaverine as substrates, Vmax: 97.3 pkat Pisum sativum
1.4.3.22 0.33
-
 N-methylputrescine pH 7.9, 30°C, lowest KM-value compared with putrescine or cadaverine as substrates, Vmax: 90.4 pkat Hyoscyamus niger
1.4.3.22 0.82
-
 N-methylputrescine pH 7.9, 30°C, lowest KM-value compared with putrescine or cadaverine as substrates, Vmax: 23.3 pkat Sus scrofa
1.4.3.22 1.22
-
 N-methylputrescine pH 7.9, 30°C, highest KM-value compared with putrescine or cadaverine as substrates, Vmax: 93.5 pkat Pisum sativum
1.4.3.22 2.85
-
 1,4-diaminobutane pH 7.9, 30°C, higher KM-value compared with N-methylputrescine or cadaverine as substrates, Vmax: 82.7 pkat, specifiity constant 11% of the constant for N-methylputrescine Hyoscyamus niger
1.4.3.22 6.25
-
 cadaverine pH 7.9, 30°C, higher KM-value compared with N-putrescine or cadaverine as substrates, Vmax: 19.6 pkat, specifiity constant 1% of the constant for N-methylputrescine Hyoscyamus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.22 66000
-
 2 * 66000, SDS-PAGE Hyoscyamus niger
1.4.3.22 135000
-
 gel filtration Hyoscyamus niger

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.22 Hyoscyamus niger
-
-
-
1.4.3.22 Pisum sativum
-
-
-
1.4.3.22 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.22 partially Hyoscyamus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.22 epicotyl
-
 Pisum sativum
-
1.4.3.22 kidney
-
 Sus scrofa
-
1.4.3.22 root culture
-
 Hyoscyamus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.22 0.0326
-
 partially purified enzyme, N-methylpurtrescine as substrate, pH 7.9, 30°C Hyoscyamus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.22 1,3-diaminopropane + H2O + O2 30% activity compared to activity with N-methylputrescine Hyoscyamus niger 3-aminopropanal + NH3 + H2O2
-
 ?
1.4.3.22 1,3-diaminopropane + H2O + O2 30% activity compared to activity with putrescine Sus scrofa 3-aminopropanal + NH3 + H2O2
-
 ?
1.4.3.22 1,4-diaminobutane + H2O + O2 best substrate Sus scrofa 4-aminobutanal + NH3 + H2O2
-
 ?
1.4.3.22 1,4-diaminobutane + H2O + O2 best substrate Pisum sativum 4-aminobutanal + NH3 + H2O2
-
 ?
1.4.3.22 1,4-diaminobutane + H2O + O2 50% activity compared to activity with N-methylputrescine Hyoscyamus niger 4-aminobutanal + NH3 + H2O2
-
 ?
1.4.3.22 cadaverine + H2O + O2 30% activity compared to activity with N-methylputrescine Hyoscyamus niger 5-aminopentanal + NH3 + H2O2
-
 ?
1.4.3.22 cadaverine + H2O + O2 75% activity compared to activity with putrescine Pisum sativum 5-aminopentanal + NH3 + H2O2
-
 ?
1.4.3.22 cadaverine + H2O + O2 80% activity compared to activity with putrescine Sus scrofa 5-aminopentanal + NH3 + H2O2
-
 ?
1.4.3.22 histamine + H2O + O2 60% activity compared to activity with putrescine Sus scrofa (imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
 ?
1.4.3.22 additional information low activity with histamine, n-butylamine, and 1,6-diaminohexane Hyoscyamus niger ?
-
 ?
1.4.3.22 additional information low activity with n-butylamine or 1,6-diaminohexane as substrate Sus scrofa ?
-
 ?
1.4.3.22 additional information low or no activity with 1,3-diaminopropane, N-methyl-1,3-diaminopropane, n-butylamine, 1,6-diaminohexane, and histamine Pisum sativum ?
-
 ?
1.4.3.22 N-methyl-1,3-diaminopropane + H2O + O2 40% activity compared to activity with N-methylputrescine Hyoscyamus niger N-methyl-3-aminopropanal + NH3 + H2O2
-
 ?
1.4.3.22 N-methyl-1,3-diaminopropane + H2O + O2 40% activity compared to activity with putrescine Sus scrofa N-methyl-3-aminopropanal + NH3 + H2O2
-
 ?
1.4.3.22 N-methylputrescine + H2O + O2 50% activity compared to activity with putrescine Pisum sativum 4-(methylamino)butanal + NH3 + H2O2
-
 ?
1.4.3.22 N-methylputrescine + H2O + O2 75% activity compared to activity with putrescine Sus scrofa 4-(methylamino)butanal + NH3 + H2O2
-
 ?
1.4.3.22 N-methylputrescine + H2O + O2 best substrate. Specificity for N-methylated diamines enable the Hyoscyamine enzyme to function specifically in tropane alkaloid biosynthesis Hyoscyamus niger 4-(methylamino)butanal + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.22 dimer 2 * 66000, SDS-PAGE Hyoscyamus niger

Synonyms

EC Number Synonyms Comment Organism
1.4.3.22 DAO
-
 Hyoscyamus niger
1.4.3.22 EC 1.4.3.6 formerly Hyoscyamus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.22 30
-
 assay at Hyoscyamus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.22 7.5 8 measured over the range of 6.0 to 9.0, with N-methylputrescine as substrate Hyoscyamus niger
1.4.3.22 7.9
-
 assay at Hyoscyamus niger

General Information

EC Number General Information Comment Organism
1.4.3.22 physiological function root cultures of Hyoscyamus niger produce nicotine and tropane alkaloids by the same early biosynthetic pathway in which the DAO functions to convert N-methylputrescine to methylpyrrolinium cation. There is no close correlaion between alkaloid production and DAO activity. The biosynthesis of nicotine and tropane alkaloids are not regulated directly by the DAO activities. Specificty for N-methylated diamines enable the Hyoscyamine enzyme to function specifically in tropane alkaloid biosynthesis Hyoscyamus niger