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Literature summary extracted from

  • Widiez, T.; Hartman, T.G.; Dudai, N.; Yan, Q.; Lawton, M.; Havkin-Frenkel, D.; Belanger, F.C.
    Functional characterization of two new members of the caffeoyl CoA O-methyltransferase-like gene family from Vanilla planifolia reveals a new class of plastid-localized O-methyltransferases (2011), Plant Mol. Biol., 76, 475-488.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.104 expressed in Escherichia coli as a His-tagged fusion protein Vanilla planifolia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.104 0.109
-
 tricetin pH 7.5, 30°C Vanilla planifolia
2.1.1.104 0.158
-
 tricetin pH 7.5, 30°C Vanilla planifolia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.104 27930
-
 calculated from cDNA Vanilla planifolia
2.1.1.104 33220
-
 calculated from cDNA Vanilla planifolia

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.104 Vanilla planifolia F2YP45
-
-
2.1.1.104 Vanilla planifolia F2YP46
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.104 using Ni-NTA chromatography Vanilla planifolia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.104 leaf high expression Vanilla planifolia
-
2.1.1.104 pod low expression Vanilla planifolia
-
2.1.1.104 pod highly expressed in the hair cells of the developing pod Vanilla planifolia
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.104 additional information enzyme prefers as a substrate the flavone tricetin. Enzyme shows a lower activity with vanillin precursor, 3,4-dihydroxybenzaldehyde, than with tricetin Vanilla planifolia ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + 3,4-dihydroxybenzaldehyde moderate activity Vanilla planifolia ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde low activity Vanilla planifolia ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde moderate activity Vanilla planifolia ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + 5-hydroxyferulic acid high activity Vanilla planifolia ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + 5-hydroxyferulic acid ethyl ester high activity Vanilla planifolia ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeic acid low activity Vanilla planifolia ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeic acid ethyl ester low activity Vanilla planifolia ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeic acid ethyl ester moderate activity Vanilla planifolia ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA moderate activity Vanilla planifolia S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoylaldehyde low activity Vanilla planifolia ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + myricetin high activity Vanilla planifolia ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + quercetin moderate activity Vanilla planifolia ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + tricetin highest activity Vanilla planifolia S-adenosyl-L-homocysteine + selgin tricin, also identified as product ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.104 caffeoyl CoA O-methyltransferase
-
 Vanilla planifolia
2.1.1.104 Vp-OMT4
-
 Vanilla planifolia
2.1.1.104 Vp-OMT5
-
 Vanilla planifolia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.104 30
-
 assay at Vanilla planifolia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.104 0.011
-
 tricetin pH 7.5, 30°C Vanilla planifolia
2.1.1.104 0.0913
-
 tricetin pH 7.5, 30°C Vanilla planifolia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.104 7.5
-
 assay at Vanilla planifolia

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.104 0.069
-
 tricetin pH 7.5, 30°C Vanilla planifolia
2.1.1.104 0.838
-
 tricetin pH 7.5, 30°C Vanilla planifolia