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Literature summary extracted from

  • Kim, I.; Kim, B.; Kim, M.; Ahn, J.
    Characterization of hydroxycinnamoyltransferase from rice and its application for biological synthesis of hydroxycinnamoyl glycerols (2012), Phytochemistry, 76, 25-31.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.133 gene OsHCT4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as soluble GST-tagged enzyme using vector pGEX 5X-1 in Escherichia coli strain BL21(DE3) Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.133 0.0601
-
 4-coumaroyl-CoA pH 7.5, 37°C, with glycerol Oryza sativa
2.3.1.133 0.0721
-
 feruloyl-CoA pH 7.5, 37°C, with glycerol Oryza sativa
2.3.1.133 0.0859
-
 glycerol pH 7.5, 37°C, with feruloyl-CoA Oryza sativa
2.3.1.133 0.102
-
 caffeoyl-CoA pH 7.5, 37°C, with glycerol Oryza sativa
2.3.1.133 0.1558
-
 shikimate pH 7.5, 37°C, with feruloyl-CoA Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.133 additional information Oryza sativa no determination of the in vivo substrate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.133 Oryza sativa
-
 gene OsHCT4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.133 soluble recombinant GST-tagged OsHCT4 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.133 4-coumaroyl-CoA + glycerol
-
 Oryza sativa CoA + 2-O-(4-coumaroyl)glycerol + 1-O-(4-coumaroyl)glycerol
-
 ?
2.3.1.133 4-coumaroyl-CoA + shikimate
-
 Oryza sativa CoA + 5-O-(4-coumaroyl)shikimate
-
 ?
2.3.1.133 caffeoyl-CoA + glycerol
-
 Oryza sativa CoA + ?
-
 ?
2.3.1.133 caffeoyl-CoA + shikimate
-
 Oryza sativa CoA + 5-O-caffeoylshikimate
-
 ?
2.3.1.133 feruloyl-CoA + glycerol
-
 Oryza sativa CoA + ?
-
 ?
2.3.1.133 feruloyl-CoA + shikimate
-
 Oryza sativa CoA + 5-O-feruloylshikimate
-
 ?
2.3.1.133 additional information no determination of the in vivo substrate Oryza sativa ?
-
 ?
2.3.1.133 additional information OsHCT4 mediates the trans-esterification of glycerol as well as shikimic acid in the presence of hydroxycinnamoyl-CoA in vitro, reaction product structure determination by NMR Oryza sativa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.133 HCS/QT
-
 Oryza sativa
2.3.1.133 hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyltransferase
-
 Oryza sativa
2.3.1.133 OsHCT4
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.133 37
-
 assay at Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.133 7.5
-
 assay at Oryza sativa

General Information

EC Number General Information Comment Organism
2.3.1.133 evolution OsHCT1 and OsHCT2 are members of the HCS/QT family, while OsHCT3 and OsHCT4 belong to a distinct group. OsHCT3 and OsHCT4 may employ a different acyl group acceptor Oryza sativa