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Literature summary extracted from

  • Toda, H.; Itoh, N.
    Isolation and characterization of a gene encoding a S-adenosyl-L-methionine-dependent halide/thiol methyltransferase (HTMT) from the marine diatom Phaeodactylum tricornutum: Biogenic mechanism of CH3I emissions in oceans (2011), Phytochemistry, 72, 337-343.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.9 His-tagged protein expressed in Escherichia coli BL21(DE3), together with chaperone-coding genes (plasmid pG-KJE8) Phaeodactylum tricornutum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.9 7.9
-
 thiocyanate pH 7.0, 30°C Phaeodactylum tricornutum
2.1.1.9 9.9
-
 SH- pH 7.0, 30°C Phaeodactylum tricornutum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.9 30000
-
 gel filtration, His-tagged protein Phaeodactylum tricornutum
2.1.1.9 34000
-
 1 * 34000, SDS-PAGE, His-tagged protein Phaeodactylum tricornutum

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.9 Phaeodactylum tricornutum B7FWR0
-
-
2.1.1.9 Phaeodactylum tricornutum CCAP 1055/1 B7FWR0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.9 immobilized metal ion affinity chromatography (Ni2+) Phaeodactylum tricornutum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.9 0.1043
-
 substrate iodometane, pH 7.0, 30°C Phaeodactylum tricornutum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.9 S-adenosyl-L-methionine + thiocyanate
-
 Phaeodactylum tricornutum S-adenosyl-L-homocysteine + methyl thiocyanate
-
 ?
2.1.1.9 S-adenosyl-L-methionine + thiocyanate
-
 Phaeodactylum tricornutum CCAP 1055/1 S-adenosyl-L-homocysteine + methyl thiocyanate
-
 ?
2.1.1.9 SH- + S-adenosyl-L-methionine
-
 Phaeodactylum tricornutum CH3SH + S-adenosyl-L-homocysteine
-
 ?
2.1.1.9 SH- + S-adenosyl-L-methionine
-
 Phaeodactylum tricornutum CCAP 1055/1 CH3SH + S-adenosyl-L-homocysteine
-
 ?
2.1.1.9 thiocyanate + S-adenosyl-L-methionine
-
 Phaeodactylum tricornutum methyl thiocyanate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.9 thiocyanate + S-adenosyl-L-methionine
-
 Phaeodactylum tricornutum CCAP 1055/1 methyl thiocyanate + S-adenosyl-L-homocysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.9 monomer 1 * 34000, SDS-PAGE, His-tagged protein Phaeodactylum tricornutum

Synonyms

EC Number Synonyms Comment Organism
2.1.1.9 S-adenosyl-L-methionine-dependent halide/thiol methyltransferase
-
 Phaeodactylum tricornutum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.9 0.0102
-
 thiocyanate pH 7.0, 30°C Phaeodactylum tricornutum
2.1.1.9 0.0381
-
 SH- pH 7.0, 30°C Phaeodactylum tricornutum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.9 7
-
 50% activity at pH 6.0 or pH 8.5 Phaeodactylum tricornutum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.9 0.00129
-
 SCN- pH 7.0, 30°C Phaeodactylum tricornutum
2.1.1.9 0.00385
-
 SH- pH 7.0, 30°C Phaeodactylum tricornutum