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Literature summary extracted from

  • Matuschek, M.; Wallwey, C.; Xie, X.; Li, S.M.
    New insights into ergot alkaloid biosynthesis in Claviceps purpurea: an agroclavine synthase EasG catalyses, via a non-enzymatic adduct with reduced glutathione, the conversion of chanoclavine-I aldehyde to agroclavine (2011), Org. Biomol. Chem., 9, 4328-4335.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.46 reduced glutathione required for activity Claviceps purpurea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.46 expressed in Escherichia coli BXL1 Blue MRF’ cells Claviceps purpurea
1.5.1.46 expressed in Escherichia coli strain XL1 Blue MRF’ Claviceps purpurea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.46 30000
-
 1 * 30000, His-tagged enzyme, SDS-PAGE Claviceps purpurea
1.5.1.46 30000
-
 x * 30000, His-tagged enzyme, SDS-PAGE Claviceps purpurea
1.5.1.46 31900
-
 1 * 31900, native enzyme, calculated from amino acid sequence Claviceps purpurea
1.5.1.46 31900
-
 x * 31900, calculated from amino acid sequence Claviceps purpurea
1.5.1.46 33300
-
 1 * 33300, His-tagged enzyme, calculated from amino acid sequence Claviceps purpurea

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.44 Aspergillus fumigatus
-
-
-
1.5.1.46 Claviceps purpurea
-
-
-
1.5.1.46 Claviceps purpurea P0CT21
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.46 Ni-NTA agarose column chromatography Claviceps purpurea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.44 chanoclavine-I aldehyde + NADH + H+ this reaction occurs in the presence of the old yellow enzyme FgaOx3. Together with GSH and NADPH, the enzyme also catalyses the conversion of chanoclavine-I aldehyde to agroclavine in the absence of the old yellow enzyme FgaOx3 Aspergillus fumigatus festuclavine + NAD+
-
 ?
1.5.1.46 chanoclavine-I aldehyde + NADH + H+ 54% conversion compared to NADPH, in the presence of reduced glutathione Claviceps purpurea agroclavine + NAD+
-
 ?
1.5.1.46 chanoclavine-I aldehyde + NADH + H+ the reaction occurs only in the presence of reduced glutathione (conversion rate of 54%) Claviceps purpurea agroclavine + NAD+
-
 ?
1.5.1.46 chanoclavine-I aldehyde + NADPH + H+ the reaction occurs only in the presence of reduced glutathione or beta-mercaptoethanol or dithiothreitol Claviceps purpurea agroclavine + NADP+
-
 ?
1.5.1.46 chanoclavine-I aldehyde + NADPH + H+ the reaction occurs only in the presence of the thiolation agents reduced glutathione (conversion rate of 59%) or 2-mercaptoethanol (conversion rate of 99%) and dithiothreitol (conversion rate of 67%). No product formation is detected in the incubation mixture with L-ascorbic acid instead of GSH Claviceps purpurea agroclavine + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.46 ? x * 30000, His-tagged enzyme, SDS-PAGE Claviceps purpurea
1.5.1.46 ? x * 31900, calculated from amino acid sequence Claviceps purpurea
1.5.1.46 monomer 1 * 30000, His-tagged enzyme, SDS-PAGE Claviceps purpurea
1.5.1.46 monomer 1 * 31900, native enzyme, calculated from amino acid sequence Claviceps purpurea
1.5.1.46 monomer 1 * 33300, His-tagged enzyme, calculated from amino acid sequence Claviceps purpurea

Synonyms

EC Number Synonyms Comment Organism
1.5.1.44 festuclavine synthase
-
 Aspergillus fumigatus
1.5.1.44 FgaFS
-
 Aspergillus fumigatus
1.5.1.46 agroclavine synthase
-
 Claviceps purpurea
1.5.1.46 EasG
-
 Claviceps purpurea

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.46 NADH
-
 Claviceps purpurea
1.5.1.46 NADPH
-
 Claviceps purpurea