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Literature summary extracted from

  • Moinuddin, S.G.; Jourdes, M.; Laskar, D.D.; Ki, C.; Cardenas, C.L.; Kim, K.W.; Zhang, D.; Davin, L.B.; Lewis, N.G.
    Insights into lignin primary structure and deconstruction from Arabidopsis thaliana COMT (caffeic acid O-methyl transferase) mutant Atomt1 (2010), Org. Biomol. Chem., 8, 3928-3946.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.68 expressed in Escherichia coli Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.68 0.0179
-
 5-hydroxyconiferyl aldehyde pH 7.5, temperature not specified in the publication Arabidopsis thaliana
2.1.1.68 0.0197
-
 caffeoyl aldehyde pH 7.5, temperature not specified in the publication Arabidopsis thaliana
2.1.1.68 0.0237
-
 quercetin pH 7.5, temperature not specified in the publication Arabidopsis thaliana
2.1.1.68 0.0242
-
 caffeic acid pH 7.5, temperature not specified in the publication Arabidopsis thaliana
2.1.1.68 0.0316
-
 5-hydroxyconiferyl alcohol pH 7.5, temperature not specified in the publication Arabidopsis thaliana
2.1.1.68 0.032
-
 5-hydroxyferulic acid pH 7.5, temperature not specified in the publication Arabidopsis thaliana
2.1.1.68 0.0515
-
 caffeoyl alcohol pH 7.5, temperature not specified in the publication Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.68 Arabidopsis thaliana
-
 17 genes are annotated as putative COMTs in Arabidopsis
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.68 using affinity chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.68 S-adenosyl-L-methionine + 5-hydroxyconiferyl alcohol
-
 Arabidopsis thaliana ?
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 5-hydroxyconiferyl aldehyde
-
 Arabidopsis thaliana ?
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 5-hydroxyferulic acid
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + sinapic acid
-
 ?
2.1.1.68 S-adenosyl-L-methionine + caffeic acid
-
 Arabidopsis thaliana ?
-
 ?
2.1.1.68 S-adenosyl-L-methionine + caffeoyl alcohol
-
 Arabidopsis thaliana ?
-
 ?
2.1.1.68 S-adenosyl-L-methionine + caffeoyl aldehyde
-
 Arabidopsis thaliana ?
-
 ?
2.1.1.68 S-adenosyl-L-methionine + quercetin
-
 Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.68 AtCOMT1
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.68 0.16
-
 quercetin pH 7.5, temperature not specified in the publication Arabidopsis thaliana
2.1.1.68 0.62
-
 caffeic acid pH 7.5, temperature not specified in the publication Arabidopsis thaliana
2.1.1.68 1.3
-
 5-hydroxyferulic acid pH 7.5, temperature not specified in the publication Arabidopsis thaliana
2.1.1.68 1.52
-
 caffeoyl alcohol pH 7.5, temperature not specified in the publication Arabidopsis thaliana
2.1.1.68 1.55
-
 caffeoyl aldehyde pH 7.5, temperature not specified in the publication Arabidopsis thaliana
2.1.1.68 2.23
-
 5-hydroxyconiferyl alcohol pH 7.5, temperature not specified in the publication Arabidopsis thaliana
2.1.1.68 2.85
-
 5-hydroxyconiferyl aldehyde pH 7.5, temperature not specified in the publication Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.68 7.5
-
 assay at Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.1.1.68 malfunction Arabidopsis COMT1 knockout mutant line Atomt1 lignin differs from native lignin in wild type plants, in terms of sinapyl (S) alcohol-derived substructures in fiber cell walls being substituted by 5-hydroxyconiferyl alcohol (5OHG)-derived moieties Arabidopsis thaliana